伊氏锥虫的随机扩增多态性DNA指纹分析
Random amplification of polymorphic DNA fingerprinting of Trypansoma evansi.
作者信息
Basagoudanavar S H, Rao J R, Singh R K, Butchaiah G
机构信息
National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar.
出版信息
Vet Res Commun. 1999 Jun;23(4):249-55. doi: 10.1023/a:1006205327299.
The total genomic DNAs from Trypanosoma evansi isolates of bubaline, equine and cameline origin were amplified by polymerase chain reaction using eight arbitrarily selected 10-base primers. Informative band patterns were obtained for all isolates analysed. Depending upon the T. evansi isolate primer combination, between 1 and 11 reproducible DNA fingerprints of 205 to 3016 bp were amplified, suggesting minor and major differences in their RAPD (random amplified polymorphic DNA) profiles.
使用8个任意选择的10碱基引物，通过聚合酶链反应扩增了来自水牛、马和骆驼源伊氏锥虫分离株的全基因组DNA。对所有分析的分离株都获得了信息丰富的条带模式。根据伊氏锥虫分离株的引物组合，扩增出了1至11条205至3016 bp的可重复DNA指纹，表明它们的随机扩增多态性DNA（RAPD）图谱存在微小和显著差异。
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