Lun Zhao-Rong, Li An-Xing, Chen Xiao-Guang, Lu Li-Xin, Zhu Xing-Quan
Center for Parasitic Organisms, School of Life Sciences, Zhongshan University, Guangzhou 510275, PR China,
Parasitol Res. 2004 Mar;92(4):335-40. doi: 10.1007/s00436-003-1054-8. Epub 2004 Jan 16.
A total of 20 random primers (10-mers) were used to amplify RAPD markers from the genomic DNA of four Trypanosoma brucei stocks from East and West Africa, four T. evansi stocks from Africa, Asia and South America and one T. equiperdum stock from Asia. Between 65 and 88 reproducible fragments ranging from 0.25 to 2.15 kb were generated from these stocks depending on the stock/primer combination. The similarity coefficient (SC) among the stocks of T. brucei from Kenya, Nigeria, Tanzania and Zambia ranged from 62.9% to 74.0% (average: 67.6%). The SC among the stocks of T. evansi from Kenya, China and Brazil was 76.4%-95.5% (average: 86.4%), while the SC between T. evansi stock from China and Brazil was 95.5%. For T. evansi and T. equiperdum, the SC among the stocks ranged from 81.2% to 94.4% (average: 87.6%). As for the SC among the stocks of T. brucei and T. evansi, it was found to be from 54.7% to 80.3% (average: 68.0%) and the SC among stocks of T. brucei and T. equiperdum was from 59.4% to 76.9% (average: 68.1%). Our results indicate that the stocks of T. evansi from China and from Brazil are more closely related to the stock of T. equiperdum from China than to the stocks of T. evansi isolated from Kenya and to the stocks of T. brucei. In addition, our results further support the hypothesis that T. evansi stocks from China and Brazil could have arisen from a single lineage. The possible evolution of T. evansi and T. equiperdum is also discussed.
总共使用了20种随机引物（10聚体），从来自东非和西非的4株布氏锥虫、来自非洲、亚洲和南美洲的4株伊氏锥虫以及来自亚洲的1株马媾疫锥虫的基因组DNA中扩增RAPD标记。根据菌株/引物组合的不同，这些菌株产生了65至88个可重复片段，片段大小在0.25至2.15 kb之间。来自肯尼亚、尼日利亚、坦桑尼亚和赞比亚的布氏锥虫菌株之间的相似系数（SC）在62.9%至74.0%之间（平均为67.6%）。来自肯尼亚、中国和巴西的伊氏锥虫菌株之间的SC为76.4% - 95.5%（平均为86.4%），而来自中国和巴西的伊氏锥虫菌株之间的SC为95.5%。对于伊氏锥虫和马媾疫锥虫，菌株之间的SC在81.2%至94.4%之间（平均为87.6%）。至于布氏锥虫和伊氏锥虫菌株之间的SC，发现其在54.7%至80.3%之间（平均为68.0%），布氏锥虫和马媾疫锥虫菌株之间的SC在59.4%至76.9%之间（平均为68.1%）。我们的结果表明，来自中国和巴西的伊氏锥虫菌株与来自中国的马媾疫锥虫菌株的关系，比与从肯尼亚分离的伊氏锥虫菌株以及布氏锥虫菌株的关系更为密切。此外，我们的结果进一步支持了这样的假设，即来自中国和巴西的伊氏锥虫菌株可能起源于单一谱系。文中还讨论了伊氏锥虫和马媾疫锥虫可能的进化情况。
