Matsubara K, Ochi H, Kitagawa H, Yamanaka K, Kusanagi Y, Ito M
Department of Obstetrics and Gynecology, School of Medicine, Ehime University, Japan.
Hypertens Pregnancy. 1999;18(1):95-106. doi: 10.3109/10641959909009614.
In order to elucidate the potential role of granulocyte-colony-stimulating factor (G-CSF) during the course of normal pregnancy and preeclampsia, we measured the serum concentrations of G-CSF in both normal pregnant women and preeclamptic patients.
Sera of 10 nonpregnant women, 34 normal pregnant women (n = 10, first trimester; n = 10, second trimester; n = 14, third trimester), 10 postpartum women, 10 mild preeclamptic patients, and 10 severe preeclamptic patients were collected. The serum concentrations of G-CSF were measured by enzyme immunoassay.
The serum level of G-CSF in normal pregnant women (third trimester: 38.3 +/- 15.3 pg/mL; mean +/- SD) was significantly increased when compared with the levels observed in nonpregnant women (20.3 +/- 10.1 pg/mL, p < 0.05), which was similar to the G-CSF concentrations in postpartum subjects (20.7 +/- 9.1 pg/mL). The mild and severe preeclamptic patients showed significantly higher levels of G-CSF (56.9 +/- 18.8 pg/mL, p < 0.05; 73.2 +/- 30.4 pg/mL, p < 0.0001, respectively) than those noted in the third trimester women. The preeclamptic patients who presented with edema had greater (p < 0.01) serum levels of G-CSF (75.5 +/- 25.0 pg/mL) compared with nonedematous patients (44.7 +/- 14.9 pg/mL). The serum levels of G-CSF significantly correlated with both weight gain (p < 0.05), diastolic blood pressure (p < 0.01), and systolic blood pressure (p < 0.01), but not with white blood cell counts.
Serum concentrations of G-CSF are increased in normal and, even more so, in preeclamptic pregnancies. Because there was no relationship between serum G-CSF concentration and the number of leukocytes, G-CSF might act not to promote the physiological leukocytosis of pregnancy, but to stimulate the function of leukocytes such as phagocytosis. Moreover, it might be that G-CSF plays important roles in the activation of granulocytes or vascular endothelial injury, which are considered to be important pathological conditions in the development of preeclampsia.
为阐明粒细胞集落刺激因子（G-CSF）在正常妊娠及子痫前期过程中的潜在作用，我们检测了正常孕妇和子痫前期患者血清中G-CSF的浓度。
收集10名未孕女性、34名正常孕妇（孕早期10名；孕中期10名；孕晚期14名）、10名产后女性、10名单纯性子痫前期患者及10名重度子痫前期患者的血清。采用酶免疫测定法检测血清中G-CSF的浓度。
与未孕女性（20.3±10.1 pg/mL）相比，正常孕妇（孕晚期：38.3±15.3 pg/mL；均值±标准差）血清G-CSF水平显著升高（p<0.05），这与产后女性血清G-CSF浓度（20.7±9.1 pg/mL）相似。单纯性和重度子痫前期患者血清G-CSF水平（分别为56.9±18.8 pg/mL，p<0.05；73.2±30.4 pg/mL，p<0.0001）显著高于孕晚期女性。有水肿的子痫前期患者血清G-CSF水平（75.5±25.0 pg/mL）显著高于无水肿患者（44.7±14.9 pg/mL）（p<0.01）。血清G-CSF水平与体重增加（p<0.05）、舒张压（p<0.01）及收缩压（p<0.01）均显著相关，但与白细胞计数无关。
正常妊娠尤其是子痫前期孕妇血清G-CSF浓度升高。由于血清G-CSF浓度与白细胞数量无关，G-CSF可能并非促进妊娠生理性白细胞增多，而是刺激白细胞功能如吞噬作用。此外，G-CSF可能在粒细胞活化或血管内皮损伤中起重要作用，而这被认为是子痫前期发生发展中的重要病理情况。
