Resnekov O
Section on Microbial Genetics, Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-2785, USA.
J Bacteriol. 1999 Sep;181(17):5384-8. doi: 10.1128/JB.181.17.5384-5388.1999.
During sporulation, the Bacillus subtilis transcription factor sigmaK is activated by regulated proteolytic processing. I have used a system that facilitates the analysis of the contributions of a modified form of the processing enzyme, SpoIVFB-GFP, and the regulatory proteins BofA and SpoIVFA to the conversion of pro-sigmaK to sigmaK. The results show that in the presence of BofA, SpoIVFA levels increase by greater than 20-fold, SpoIVFA is substantially stabilized, and pro-sigmaK processing is inhibited. In addition, enhanced accumulation of the SpoIVFA protein in the absence of BofA (achieved through the use of an ftsH null mutation) substantially inhibits pro-sigmaK processing. These results suggest that during growth, increased accumulation of the SpoIVFA protein inhibits the activity of SpoIVFB-GFP and regulates the activation of sigmaK.
在芽孢形成过程中,枯草芽孢杆菌转录因子σK通过受调控的蛋白水解加工而被激活。我使用了一个系统,该系统有助于分析加工酶的一种修饰形式SpoIVFB-GFP以及调控蛋白BofA和SpoIVFA对前体σK转化为σK的贡献。结果表明,在存在BofA的情况下,SpoIVFA水平增加超过20倍,SpoIVFA得到显著稳定,并且前体σK的加工受到抑制。此外,在不存在BofA的情况下(通过使用ftsH无效突变实现)SpoIVFA蛋白的积累增强,这基本上抑制了前体σK的加工。这些结果表明,在生长过程中,SpoIVFA蛋白积累的增加会抑制SpoIVFB-GFP的活性并调节σK的激活。