Lewis A P, Thomas P J
Advanced Technology and Informatics Unit, GlaxoWellcome Medicines Research Centre, Stevenage, United Kingdom.
Protein Sci. 1999 Feb;8(2):439-42. doi: 10.1110/ps.8.2.439.
Computer-based database searching and protein multiple sequence alignment has identified a novel clan of zinc metallopeptidases, which, by phylogenetic analysis, has been shown to contain six subfamilies. The family is characterized by four common transmembrane segments and three conserved sequence motifs. The combination of topology analysis and motif identification has detected three potential Zn2+ coordinating residues. Only two of the sequences of this novel zinc metallopeptidase clan possess any functional annotation, one of which is able to cleave its substrate within a cytosol/transmembrane segment junction. A number of observations suggest that the remaining members of this novel clan may also cleave their substrates within transmembrane segments.
基于计算机的数据库搜索和蛋白质多序列比对已鉴定出一个新的锌金属肽酶家族，通过系统发育分析表明该家族包含六个亚家族。该家族的特征是有四个常见的跨膜区段和三个保守的序列基序。拓扑分析和基序鉴定相结合已检测到三个潜在的锌离子配位残基。这个新的锌金属肽酶家族中只有两个序列有任何功能注释，其中一个能够在胞质溶胶/跨膜区段交界处切割其底物。一些观察结果表明，这个新家族的其余成员可能也在跨膜区段内切割其底物。
