Hurskainen T L, Hirohata S, Seldin M F, Apte S S
Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
J Biol Chem. 1999 Sep 3;274(36):25555-63. doi: 10.1074/jbc.274.36.25555.
We report the primary structure of three novel, putative zinc metalloproteases designated ADAM-TS5, ADAM-TS6, and ADAM-TS7. All have a similar domain organization, comprising a preproregion, a reprolysin-type catalytic domain, a disintegrin-like domain, a thrombospondin type-1 (TS) module, a cysteine-rich domain, a spacer domain without cysteine residues, and a COOH-terminal TS module. These genes are differentially regulated during mouse embryogenesis and in adult tissues, with Adamts5 highly expressed in the peri-implantation period in embryo and trophoblast. These proteins are similar to four other cognate gene products, defining a distinct family of human reprolysin-like metalloproteases, the ADAM-TS family. The other members of the family are ADAM-TS1, an inflammation-induced gene, the procollagen I/II amino-propeptide processing enzyme (PCINP, ADAM-TS2), and proteins predicted by the KIAA0366 and KIAA0688 genes (ADAM-TS3 and ADAM-TS4). Individual ADAM-TS members differ in the number of COOH-terminal TS modules, and some have unique COOH-terminal domains. The ADAM-TS genes are dispersed in human and mouse genomes.
我们报告了三种新的假定锌金属蛋白酶ADAM - TS5、ADAM - TS6和ADAM - TS7的一级结构。它们都具有相似的结构域组织，包括一个前原区域、一个类解聚素型催化结构域、一个类整合素结构域、一个血小板反应蛋白1型（TS）模块、一个富含半胱氨酸的结构域、一个不含半胱氨酸残基的间隔结构域以及一个COOH末端TS模块。这些基因在小鼠胚胎发育过程和成年组织中受到不同程度的调控，Adamts5在胚胎和滋养层的着床前期高度表达。这些蛋白质与其他四种同源基因产物相似，定义了一个独特的人类类解聚素样金属蛋白酶家族，即ADAM - TS家族。该家族的其他成员包括炎症诱导基因ADAM - TS1、原胶原I/II氨基前肽加工酶（PCINP，ADAM - TS2）以及由KIAA0366和KIAA0688基因预测的蛋白质（ADAM - TS3和ADAM - TS4）。各个ADAM - TS成员在COOH末端TS模块的数量上有所不同，并且一些成员具有独特的COOH末端结构域。ADAM - TS基因分散在人类和小鼠基因组中。
