Zochodne D W, Nguyen C
Department of Clinical Neurosciences, University of Calgary, Alberta, Canada.
J Neurol Sci. 1999 Jun 15;166(1):40-6. doi: 10.1016/s0022-510x(99)00111-2.
Microangiopathy is an important complication of diabetes mellitus and neovascularity is a feature of human diabetic retinopathy. The objective of this work was to evaluate numbers, areas and size distributions of whole nerve, endoneurial and dorsal root ganglia perfused microvessels in a detailed fashion using unfixed tissues from rats with experimental diabetes. Experimental neuropathy was studied in male Sprague-Dawley rats 12 weeks after streptozotocin or citrate buffer injection. Electrophysiological recordings of sciatic-tibial motor and caudal sensory fibers identified conduction slowing in diabetes indicating neuropathy. Diabetics had a rise in the numbers of whole nerve microvessels and endoneurial microvessels with associated rises in vessel densities and total vessel luminal areas. Increased vessel numbers in 15-30 microm diameter size ranges were particularly prominent. There was a rise in summed vascular areas in diabetics but the mean luminal area of vessels was not increased. Similar, but not significant trends were observed in a selective analysis of endoneurial vessels alone. In contrast, dorsal root ganglia microvessels were not increased in number. Early experimental diabetic neuropathy is associated with increased numbers of microvessels supplying the peripheral nerve trunk, likely representing neovascularity.
微血管病变是糖尿病的一种重要并发症，新生血管形成是人类糖尿病视网膜病变的一个特征。本研究的目的是使用实验性糖尿病大鼠的未固定组织，详细评估全神经、神经内膜和背根神经节灌注微血管的数量、面积和大小分布。在雄性Sprague-Dawley大鼠注射链脲佐菌素或柠檬酸盐缓冲液12周后研究实验性神经病变。坐骨神经-胫神经运动纤维和尾神经感觉纤维的电生理记录确定糖尿病中存在传导减慢，提示神经病变。糖尿病大鼠全神经微血管和神经内膜微血管数量增加，同时血管密度和血管总腔面积也增加。直径在15 - 30微米范围内的血管数量增加尤为显著。糖尿病大鼠的血管总面积增加，但血管的平均腔面积未增加。单独对神经内膜血管进行选择性分析时观察到类似但不显著的趋势。相比之下，背根神经节微血管数量没有增加。早期实验性糖尿病神经病变与供应周围神经干的微血管数量增加有关，这可能代表新生血管形成。
