Hauser-Kronberger C, Cheung A, Hacker G W, Graf A H, Dietze O, Frick J
Institute of Pathological Anatomy, Immunohistochemistry and Biochemistry Unit, University of Salzburg, Austria.
Peptides. 1999;20(5):539-43. doi: 10.1016/s0196-9781(99)00005-4.
In the present study, the distributions of neuropeptides in the normal human clitoris and in a clitoris from an adrenogenital syndrome (AGS) was demonstrated by immunohistochemistry (IHC). Immunohistochemical screening detected a complex network of nerve fibers containing vasoactive intestinal polypeptide (VIP), peptide histidine methionine (PHM), neuropeptide tyrosine (neuropeptide Y), C-flanking peptide of neuropeptide Y (CPON), calcitonin gene-related peptide (CGRP) and substance P immunoreactivities. Special attention was given to the VIP-related peptide helospectin, that has been detected in neuronal elements in the clitoris. No visible differences between the localization and distribution of peptidergic nerve fibers of normal and hypertrophic clitoris from AGS have been observed. Co-localization studies showed the co-existence of VIP, PHM and partly helospectin and neuropeptide Y with CPON within nerve fibers in the cavernous tissue and substance P and CGRP co-expression in nerve fibers especially underneath and within the glans clitoris.
在本研究中，通过免疫组织化学（IHC）证实了神经肽在正常人类阴蒂和肾上腺生殖综合征（AGS）患者阴蒂中的分布。免疫组织化学筛查检测到一个复杂的神经纤维网络，其含有血管活性肠肽（VIP）、肽组氨酸甲硫氨酸（PHM）、神经肽Y、神经肽Y的C端侧翼肽（CPON）、降钙素基因相关肽（CGRP）和P物质免疫反应性。特别关注了与VIP相关的肽helospectin，其已在阴蒂的神经元成分中检测到。未观察到正常阴蒂和AGS患者肥大阴蒂中肽能神经纤维的定位和分布存在明显差异。共定位研究表明，海绵体组织神经纤维内VIP、PHM以及部分helospectin和神经肽Y与CPON共存，而P物质和CGRP在神经纤维中共同表达，尤其是在阴蒂头下方和内部。
