Reimann R, Zubay G
Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
Orig Life Evol Biosph. 1999 May;29(3):229-47. doi: 10.1023/a:1006580009791.
Plausible prebiotic conditions for the phosphorylation of nucleosides by inorganic phosphate were reported by Lohrmann and Orgel in 1971. This reaction was carried out on heated dry films and promoted by urea. The major products formed were nucleoside-2:3 cyclicPs;5-NMPs and other derivatives were also formed. Minor modifications of the Lohrmann and Orgel system have resulted in the preferential formation of 5-NMPs. In this modified system a 2-fold preference for phosphorylation of the 5-OH group over the 3(2)-OH group was observed and the formation of other derivatives was minimized. The small amounts of bis compounds that were formed in this system could be quantitatively removed by selective binding to the mineral hydroxylapatite at moderate ionic strengths. It was also discovered that under hydrolytic conditions there was a 3:1 preference for removal of phosphates attached to the 3-OH group over the 5-OH group. A recycling procedure for obtaining additonal 5-NMPs from bis compounds and 3-NMPs is proposed.
1971年，洛尔曼和奥格尔报道了无机磷酸盐使核苷磷酸化的合理的益生元条件。该反应在加热的干膜上进行，并由尿素促进。形成的主要产物是核苷-2:3环状磷酸酯；还形成了5'-核苷酸（5-NMPs）和其他衍生物。对洛尔曼和奥格尔体系进行的微小改进导致了5'-核苷酸的优先形成。在这个改进的体系中，观察到5'-羟基的磷酸化相对于3'（2'）-羟基有2倍的偏好，并且其他衍生物的形成被最小化。在这个体系中形成的少量双化合物可以通过在中等离子强度下与矿物羟基磷灰石选择性结合而被定量去除。还发现，在水解条件下，相对于5'-羟基，连接在3'-羟基上的磷酸盐的去除有3:1的偏好。提出了一种从双化合物和3'-核苷酸中获得额外5'-核苷酸的循环程序。
