Haque K, Truman C, Dittmer I, Denning-Kendall P, Hows J, Bradley B
Division of Transplantation Sciences, University of Bristol, UK.
Asian Pac J Allergy Immunol. 1999 Jun;17(2):93-9.
There is speculation that high cytotoxic T lymphocyte precursor frequencies (CTLpf) correlate with poor clinical outcome of bone marrow/organ transplantation. It is also believed that human umbilical cord blood is immunologically naive, and, therefore cord blood T cells may be less able to mediate graft versus host disease than marrow-derived T cells. CTLpf were determined in peripheral blood mononuclear cells collected from healthy adults, human umbilical cord blood and renal dialysis patients who were randomly selected and entered into this study. A highly sensitive non-radioactive Europium release cytotoxicity assay was optimized and modified to carry out the CTLpf estimation by using the principle of limiting dilution analysis. The results of CTLpf in healthy adults ranged from 1/694 to 1/66,666, median 1/7,339 (n=10); cord blood ranged from 1/1,562 to 1/35,714, median 1/10,162 (n=6) and dialysis patients ranged from 1/1,054 to 1/17,857 median 1/5,208 (n=9). The results demonstrated that there is little difference of CTLpf median values between the groups, but there is a wide variation of CTLpf between individuals within a population. It suggests that this variation should be taken into account when considering CTLpf assay as pre-transplantation cross-match procedure.
有推测认为，高细胞毒性T淋巴细胞前体频率（CTLpf）与骨髓/器官移植的不良临床结果相关。也有人认为，人类脐带血在免疫上是未成熟的，因此脐带血T细胞介导移植物抗宿主病的能力可能低于骨髓来源的T细胞。对随机选取并参与本研究的健康成年人、人类脐带血和肾透析患者外周血单个核细胞中的CTLpf进行了测定。通过使用极限稀释分析原理，对一种高灵敏度非放射性铕释放细胞毒性测定法进行了优化和改进，以进行CTLpf估计。健康成年人的CTLpf结果范围为1/694至1/66,666，中位数为1/7,339（n = 10）；脐带血范围为1/1,562至1/35,714，中位数为1/10,162（n = 6）；透析患者范围为1/1,054至1/17,857，中位数为1/5,208（n = 9）。结果表明，各组之间CTLpf中位数差异不大，但群体内个体之间的CTLpf存在很大差异。这表明，在将CTLpf测定作为移植前交叉配型程序时，应考虑到这种差异。
