Richard-Lenoble D, Smith M D, Loisy M
Ann Trop Med Parasitol. 1978 Dec;72(6):553-60. doi: 10.1080/00034983.1978.11719360.
Three serological methods were examined for the diagnosis of hydatid disease: indirect haemagglutination, immuno-electrodiffusion and radioimmunoassay. The sensitivity of the three methods was similar. The principal subclass of specific anti-hydatid immunoglobulin was IgG and high levels of specific anti-IgE were found in two out of the five patients studied. The hypothesis that false negative reactions seen in patients with clinically confirmed hydatidosis may be due to the presence of circulating immune complexes was explored. Immune complexes were demonstrated in only two out of 13 patients studied and these patients did not have negative sera. It is concluded that for a satisfactory serodiagnosis of hydatid disease the radioimmunoassay and immuno-electrodiffusion should both be used and that further work should be done on the purification of hydatid antigens to improve the sensitivity of the radioimmunoassay without loss of specificity.
对三种血清学方法进行了检测，以诊断包虫病：间接血凝试验、免疫电泳扩散法和放射免疫分析法。这三种方法的敏感性相似。特异性抗包虫免疫球蛋白的主要亚类是IgG，在所研究的5例患者中有2例发现了高水平的特异性抗IgE。探讨了临床确诊为包虫病的患者出现假阴性反应可能是由于循环免疫复合物的存在这一假说。在所研究的13例患者中，仅2例检测到免疫复合物，且这些患者的血清并非阴性。结论是，为了对包虫病进行满意的血清学诊断，应同时使用放射免疫分析法和免疫电泳扩散法，并且应进一步开展包虫抗原纯化工作，以提高放射免疫分析法的敏感性而不丧失特异性。
