Sorice F, Delia S, Vullo V, Aceti A, Ferone U
Ann Sclavo. 1979 Nov-Dec;21(6):800-15.
The radioallergosorbent test (RAST) for specific IgE antibodies to Echinococcus granulosus was compared with other immunological methods in regard to sensitivity, specificity and its use as a diagnostic aid for hydatid disease. Sera used were from patients with active hydatidosis proved surgically, from persons operated for hydatidosis during the previous 2-10 years and from patients with other parasitic diseases or healthy subjects. Results with enzyme-immuno assay (ELISA), indirect haemagglutination (IHA), counter-immunoelectrophoresis (CIEP) and skin test were compared with those with RAST. The percentage of positive RAST reactions (81.2%) among the patients with active hydatidosis was slightly lower than the percentage of positive ELISA (90.6%), IHA (90.6%) and skin test (87.5%) and was superior to CIEP (75%). Among the patients with previous hydatidosis, the RAST was negative in 90% of case, whereas other serological tests were positive in a considerable proportion of cases (ELISA test, 80%; IHA test, 40%; CIEP test, 40%; skin test, 60%). A high percentage (50%) of false positive RAST reactions have been observed in sera from patients infested with other parasites. Results indicated that the RAST for IgE antibodies not be used as the only method for diagnosis of hydatidosis, but it may be employed, if serological data obtained before surgery are available for comparison, for evaluate the results of surgery and to clarify the prognosis.
就敏感性、特异性及其作为包虫病诊断辅助手段的用途而言，对细粒棘球绦虫特异性IgE抗体的放射变应原吸附试验（RAST）与其他免疫学方法进行了比较。所用血清来自经手术证实患有活动性包虫病的患者、过去2至10年接受过包虫病手术的人员以及患有其他寄生虫病的患者或健康受试者。将酶免疫测定（ELISA）、间接血凝试验（IHA）、对流免疫电泳（CIEP）和皮肤试验的结果与RAST的结果进行了比较。活动性包虫病患者中RAST阳性反应的百分比（81.2%）略低于ELISA阳性百分比（90.6%）、IHA阳性百分比（90.6%）和皮肤试验阳性百分比（87.5%），但优于CIEP（75%）。在既往有包虫病的患者中，90%的病例RAST为阴性，而其他血清学检测在相当一部分病例中呈阳性（ELISA试验，80%；IHA试验，40%；CIEP试验，40%；皮肤试验，60%）。在感染其他寄生虫的患者血清中观察到较高百分比（50%）的RAST假阳性反应。结果表明，IgE抗体的RAST不能用作诊断包虫病的唯一方法，但如果术前获得的血清学数据可供比较，则可用于评估手术结果并阐明预后。
