Bais HP, George J, Ravishankar GA
Department of Plant Cell Biotechnology, Central Food Technological Research Institute, Mysore 570 013, India
J Plant Growth Regul. 1999 Aug;18(1):33-37. doi: 10.1007/pl00007043.
The effect of polyamines (putrescine, spermidine, and spermine) was examined for growth and production of two coumarins, esculetin and esculin, in the hairy roots of chicory (Cichorium intybus L. cv. Lucknow local). Of the polyamines administered, 1.5 mm putrescine alone resulted in a 2.3-fold higher increase in the growth of hairy roots as well as in the production of esculetin and esculin, which was 3.37 times more than that of the control on day 21. The endogenous level of conjugated putrescine was more than fivefold that of free putrescine levels in untreated samples. The production of esculetin and esculin in hairy root cultures strictly correlated with growth in all of the treatments. Putrescine at 1.5 mm resulted in a greater length of primary root (18.29 +/- 1.37 cm) compared with the control (10.96 +/- 0.82 cm) and more secondary and tertiary roots. This study also provides insight into the morphogenetic changes that occur in roots in response to the external supply of polyamines.Key Words. Cichorium intybus L. cv. Lucknow local-Hairy root cultures-Polyamines-Coumarinshttp://link.springer-ny.com/link/service/journals/00344/bibs/18n1p33.html
研究了多胺（腐胺、亚精胺和精胺）对菊苣（菊苣品种勒克瑙本地种）毛状根中两种香豆素（七叶亭和七叶苷）生长和产量的影响。在所施用的多胺中，单独使用1.5 mM腐胺使毛状根的生长以及七叶亭和七叶苷的产量提高了2.3倍，在第21天时比对照高出3.37倍。在未处理的样品中，结合态腐胺的内源水平是游离腐胺水平的五倍多。在所有处理中，毛状根培养物中七叶亭和七叶苷的产量与生长密切相关。与对照（10.96±0.82 cm）相比，1.5 mM腐胺使主根长度更长（18.29±1.37 cm），侧根和三级根更多。本研究还深入了解了根中因外源供应多胺而发生的形态发生变化。关键词：菊苣品种勒克瑙本地种；毛状根培养；多胺；香豆素。http://link.springer-ny.com/link/service/journals/00344/bibs/18n1p33.html
