Bais H P, Sudha G, Suresh B, Ravishankar G A
Department of Plant Cell Biotechnology, Central Food Technological Research Institute, Mysore 570 013, India.
Indian J Exp Biol. 2001 Jun;39(6):564-71.
Effect of addition of a permeabilizing agent dimethyl sulfoxide (DMSO) and a solid adsorbent, XAD -7, on growth and coumarin production in hairy root cultures of C. intybus was studied. Continuous permeabilization of the hairy root cultures of C. intybus with DMSO has been shown to be an effective strategy for enhanced release of coumarins while preserving the root viability. DMSO at 0.2% (v/v) level showed the maximum growth and coumarin production but was less as compared to control on day 28. Treatment of cells with increasing concentrations of DMSO (0.3 - 0.6 % v/v) to hairy root cultures of C. intybus, showed an inverse relationship with growth and coumarin production. Growth and production of coumarins increased with 1% media filtrate (MF) of cultures of Phytopthora parasitica var. nicotiana treatment. It was observed that treatment with DMSO (0.2% v/v) and 1% MF of P. parasitica showed the better growth and coumarin production with an increased release of coumarins as compared to the control and other treatments. It was observed that treatment of hairy root cultures with XAD-7 resulted in lesser growth and coumarin production as compared to control during the culture period. Addition of XAD-7 along with 1% MF of P. parasitica showed enhanced growth, coumarin production and increased adsorption as compared to control and lone XAD-7 treatment. Combined addition of DMSO/XAD-7 with fungal elicitor showed synergistic response in terms of biomass and coumarin production. Excretion of coumarins in both the cases was dependent on the presence of DMSO/XAD-7. These results showed that continuous permeabilization of hairy root cultures of C. intybus by using DMSO at 0.2% (v/v) level coupled with 1% MF of P. parasitica maintained viability of tissues and produced coumarins at higher level.
研究了添加透化剂二甲基亚砜（DMSO）和固体吸附剂XAD -7对菊苣毛状根培养物生长及香豆素产量的影响。已证明用DMSO对菊苣毛状根培养物进行连续透化是增强香豆素释放同时保持根活力的有效策略。0.2%（v/v）浓度的DMSO表现出最大生长量和香豆素产量，但在第28天时与对照相比有所降低。用浓度递增的DMSO（0.3 - 0.6% v/v）处理菊苣毛状根培养物，结果显示其与生长和香豆素产量呈负相关。用寄生疫霉烟草变种培养物的1%培养基滤液（MF）处理可使香豆素的生长和产量增加。据观察，与对照和其他处理相比，用DMSO（0.2% v/v）和1%寄生疫霉MF处理表现出更好的生长和香豆素产量，且香豆素释放增加。据观察，在培养期间，用XAD -7处理毛状根培养物与对照相比，生长和香豆素产量较低。与对照和单独的XAD -7处理相比，并添加1%寄生疫霉MF，添加XAD -7可促进生长、提高香豆素产量并增加吸附。DMSO/XAD -7与真菌激发子联合添加在生物量和香豆素产量方面表现出协同反应。在这两种情况下，香豆素的分泌都依赖于DMSO/XAD -7的存在。这些结果表明，以0.2%（v/v）浓度的DMSO与1%寄生疫霉MF联合使用对菊苣毛状根培养物进行连续透化，可保持组织活力并提高香豆素产量。
