Rosenzwajg M, Fery N, Bons V, Damaj G, Gluckman E, Gluckman J
Service d'Immunologie Biologique, Hôpital de la Pitié-Salpêtrière, Paris, France.
Bone Marrow Transplant. 1999 Aug;24(4):351-4. doi: 10.1038/sj.bmt.1701931.
Human herpes virus 8 (HHV8) may be sexually transmitted, but transmission via blood cells has not yet been excluded. We used a modified immunofluorescence assay to detect Ab to HHV8 latency-associated nuclear Ag in sera of 200 allogeneic BMT recipients and their related donors. In control subjects, Ab were found in 85% of patients with AIDS-related Kaposi sarcoma (n = 52), 34% of HIV-1 infected subjects without Kaposi sarcoma (n = 56) and 9. 5% of blood donors (n = 42). Among BMT donors, 14.5% were HHV8+, while 10% of recipients were positive before, and 18% after BMT. In the 176 HHV8-negative recipients at BMT, there was no relationship between post-BMT seroconversion, which occurred in 26 cases (15%), and the donor's serological status. Of note, 10 HHV8+ recipients before BMT became negative post-BMT. Outcome of BMT was not influenced by prior HHV8 seropositivity, seroconversion or seroreversion of recipients. That HHV8 seropositivity among blood donors from the Paris area was comparable to that of BMT donors and recipients before BMT indicates that these patients had not been at risk of HHV8 by blood products received before BMT, although post-BMT HHV8 seroconversion probably corresponded to contamination by blood transfusions rather than by the BMT.
人类疱疹病毒8型（HHV8）可能通过性传播，但尚未排除通过血细胞传播的可能性。我们使用改良的免疫荧光测定法检测了200名异基因骨髓移植受者及其相关供者血清中针对HHV8潜伏相关核抗原的抗体。在对照受试者中，85%的艾滋病相关卡波西肉瘤患者（n = 52）、34%无卡波西肉瘤的HIV-1感染受试者（n = 56）以及9.5%的献血者（n = 42）体内发现了抗体。在骨髓移植供者中，14.5%为HHV8阳性，而10%的受者在骨髓移植前呈阳性，骨髓移植后为18%。在176名骨髓移植时HHV8阴性的受者中，26例（15%）出现的骨髓移植后血清转化与供者的血清学状态之间没有关联。值得注意的是，10名骨髓移植前HHV8阳性的受者在骨髓移植后转为阴性。骨髓移植的结果不受受者先前HHV8血清阳性、血清转化或血清逆转的影响。巴黎地区献血者中的HHV8血清阳性率与骨髓移植供者以及骨髓移植前受者的血清阳性率相当，这表明这些患者在骨髓移植前接受血液制品时没有感染HHV8的风险，尽管骨髓移植后HHV8血清转化可能是由于输血污染而非骨髓移植所致。
