Mauer D J, Rowley W A
Department of Entomology, Iowa State University, Ames 50011-3222, USA.
J Med Entomol. 1999 Jul;36(4):503-7. doi: 10.1093/jmedent/36.4.503.
Methylene chloride extracts of flowers from 4 plants (common milkweed, Asclepias syriaca L; ox-eye daisy, Leucanthemum vulgare Lamarck; Canada goldenrod, Solidago canadensis L.; and yarrow, Achillea millefolium L.) were tested in a dual-port olfactometer for attractiveness to female northern house mosquitoes, Culex pipiens pipiens L. Fifty milligrams of extract was used for each test, which was equivalent to the amount of extract from flowers of 0.58 common milkweed inflorescences, 1.75 ox-eye daisy flower heads, 0.33 Canada goldenrod plants, or 3.14 yarrow plants. Significantly more Cx. pipiens responded to the common milkweed flower extract than to a control. Ox-eye daisy, Canada goldenrod and yarrow flower extracts were not significantly attractive to this species. A headspace sample of the common milkweed extract was collected by solid phase microextraction and analyzed by gas chromatography-mass spectrometry. The most abundant compounds in the headspace sample were 2-phenylethanol and benzyl alcohol. Analysis of the common milkweed extract and standards of 2-phenylethanol and benzyl alcohol by gas chromatography-flame ionization detection confirmed that these compounds were present in the extract. Cx. pipiens did not respond to synthetic blends of these 2 compounds when tested in a dual-port olfactometer.
对4种植物（普通马利筋，即叙利亚马利筋；滨菊，即法国菊；加拿大一枝黄花；欧蓍草）花朵的二氯甲烷提取物在双端口嗅觉仪中进行了测试，以检测其对雌性北方家蚊（致倦库蚊）的吸引力。每次测试使用50毫克提取物，这相当于0.58个普通马利筋花序、1.75个滨菊花头、0.33株加拿大一枝黄花或3.14株欧蓍草花朵的提取物量。与对照相比，显著更多的致倦库蚊对普通马利筋花提取物有反应。滨菊、加拿大一枝黄花和欧蓍草花提取物对该物种没有显著吸引力。通过固相微萃取收集普通马利筋提取物的顶空样品，并通过气相色谱 - 质谱联用仪进行分析。顶空样品中含量最丰富的化合物是2 - 苯乙醇和苯甲醇。通过气相色谱 - 火焰离子化检测对普通马利筋提取物以及2 - 苯乙醇和苯甲醇标准品进行分析，证实这些化合物存在于提取物中。当在双端口嗅觉仪中进行测试时，致倦库蚊对这两种化合物的合成混合物没有反应。
