水稻赤霉素不敏感矮化突变体基因Dwarf 1编码GTP结合蛋白的α亚基。
Rice gibberellin-insensitive dwarf mutant gene Dwarf 1 encodes the alpha-subunit of GTP-binding protein.
作者信息
Ashikari M, Wu J, Yano M, Sasaki T, Yoshimura A
机构信息
Plant Breeding Laboratory, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
出版信息
Proc Natl Acad Sci U S A. 1999 Aug 31;96(18):10284-9. doi: 10.1073/pnas.96.18.10284.
A rice Dwarf 1 gene was identified by using a map-based cloning strategy. Its recessive mutant allele confers a dwarf phenotype. Linkage analysis revealed that a cDNA encoding the alpha-subunit of GTP-binding protein cosegregated with d1 in 3,185 d1 segregants. Southern hybridization analysis with this cDNA as a probe showed different band patterns in several d1 mutant lines. In at least four independent d1 mutants, no gene transcript was observed by Northern hybridization analysis. Sequencing analysis revealed that an 833-bp deletion had occurred in one of the mutant alleles, which resulted in an inability to express GTP-binding protein. A transgenic d1 mutant with GTP-binding protein gene restored the normal phenotype. We conclude that the rice Dwarf 1 gene encodes GTP-binding protein and that the protein plays an important role in plant growth and development. Because the d1 mutant is classified as gibberellin-insensitive, we suggest that the GTP-binding protein might be associated with gibberellin signal transduction.
利用图位克隆策略鉴定出一个水稻矮秆1基因。其隐性突变等位基因导致矮化表型。连锁分析表明，在3185个d1分离群体中，一个编码GTP结合蛋白α亚基的cDNA与d1共分离。以该cDNA为探针进行的Southern杂交分析显示，在几个d1突变系中出现了不同的条带模式。在至少四个独立的d1突变体中，Northern杂交分析未观察到基因转录本。测序分析表明，一个突变等位基因发生了833 bp的缺失，导致无法表达GTP结合蛋白。携带GTP结合蛋白基因的转基因d1突变体恢复了正常表型。我们得出结论，水稻矮秆1基因编码GTP结合蛋白，该蛋白在植物生长发育中起重要作用。由于d1突变体被归类为对赤霉素不敏感，我们认为GTP结合蛋白可能与赤霉素信号转导有关。
Zotero 插件