大鼠巨球蛋白在荷瘤大鼠体内的组织分布

Tissue distribution of rat macroglobulins in tumour-bearing rats.

作者信息

Maltseva N V, Zorina R M, Mingaljev N V, Krajushkina N A, Chirikova T S, Zorin N A

机构信息

Central Research Laboratory, Postgraduate Physician Training Institute, Novokuznetsk, Russia.

出版信息

Int J Exp Pathol. 1999 Apr;80(2):105-8. doi: 10.1046/j.1365-2613.1999.00103.x.

DOI:10.1046/j.1365-2613.1999.00103.x

PMID:10469265

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2517758/

Abstract

Rat macroglobulins were determined in blood sera and extracts of tissues of intact rats and rats with Walker carcinoma by rocket immunoelectrophoresis. The serum levels of alpha1-macroglobulin (alpha1MG) alpha2-macroglobulin (alpha2MG) and pregnancy-associated alpha1-glycoprotein (alpha1PAG) were 1.86 +/- 0.07 mg/ml, 0.12 +/- 0. 02 mg/ml and 18.32 +/- 4.07 AU/ml respectively in control rats. Maximum concentrations of alpha1MG were found in heart, lung and spleen and lesser quantities were in liver and thymus, while alpha2MG and alpha1PAG were not found at all in tissue extracts from control rats. Serum alpha2MG and alpha1PAG concentrations increased more than 30-fold in tumour-bearing rats compared to control animals, while alpha1MG serum concentration was little changed. Increases in all three macroglobulins occurred in the tissues of tumour-bearing rats, particularly alpha1PAG. The tissue concentrations of alpha1MG and alpha2MG were similar and the tissue distribution was also similar with highest concentrations in heart and lung. Considerable quantities of the proteins were found in the tumour and part of peritoneum which made contact with the tumour. Changes in the protein concentrations in serum and tissue extracts of tumour-bearing rats suggest that all members of rat macroglobulin family are disturbed during the development of the Walker carcinoma, though only alpha2MG and alpha1PAG were substantially elevated.

摘要

采用火箭免疫电泳法测定了正常大鼠和患有沃克癌大鼠的血清及组织提取物中的大鼠巨球蛋白。对照大鼠血清中α1-巨球蛋白（α1MG）、α2-巨球蛋白（α2MG）和妊娠相关α1-糖蛋白（α1PAG）的水平分别为1.86±0.07mg/ml、0.12±0.02mg/ml和18.32±4.07AU/ml。在对照大鼠的心脏、肺和脾脏中发现α1MG的浓度最高，在肝脏和胸腺中的含量较少，而在对照大鼠的组织提取物中根本未发现α2MG和α1PAG。与对照动物相比，荷瘤大鼠血清中α2MG和α1PAG的浓度增加了30多倍，而α1MG的血清浓度变化不大。在荷瘤大鼠的组织中，所有三种巨球蛋白均有增加，尤其是α1PAG。α1MG和α2MG的组织浓度相似，组织分布也相似，在心脏和肺中的浓度最高。在肿瘤及与肿瘤接触的部分腹膜中发现了大量的这些蛋白质。荷瘤大鼠血清和组织提取物中蛋白质浓度的变化表明，在沃克癌的发生发展过程中，大鼠巨球蛋白家族的所有成员均受到干扰，尽管只有α2MG和α1PAG显著升高。