莫里斯肝癌切片与大鼠肝脏合成及分泌血浆白蛋白、纤维蛋白原和α2-巨球蛋白的比较
Comparison of synthesis and secretion of plasma albumin, fibrinogen and alpha 2-macroglobulin by slices of Morris hepatomas and rat liver.
作者信息
Koj A
出版信息
Br J Exp Pathol. 1980 Jun;61(3):332-8.
Abstract
Slices of rat liver or Morris Hepatomas 5123 GVI and 7777 were incubated for 2 h with 14C-leucine. Newly synthesized labelled proteins were determined independently in the medium and tissue homogenate, total protein after precipitation with trichloro-acetic acid, and albumin, fibrinogen and alpha 2-macroglobulin after immuno-precipitation with monospecific antisera. In comparison with control rat liver, synthesis and secretion of albumin were reduced four- to five-fold in the 2 heptomas examined. On the other hand, synthesis and secretio of fibrinogen and alpha 2-macroglobulin in Hepatomas 5123 GVI annd 7777 were similar to rat liver, especially to the liver isolated from rats with turpentine-induced local inflammation.
摘要
将大鼠肝脏切片或莫里斯肝癌5123 GVI和7777与14C-亮氨酸一起孵育2小时。分别在培养基和组织匀浆中测定新合成的标记蛋白质，用三氯乙酸沉淀后的总蛋白质，以及用单特异性抗血清免疫沉淀后的白蛋白、纤维蛋白原和α2-巨球蛋白。与对照大鼠肝脏相比，在所检测的两种肝癌中，白蛋白的合成和分泌减少了四至五倍。另一方面，肝癌5123 GVI和7777中纤维蛋白原和α2-巨球蛋白的合成和分泌与大鼠肝脏相似，尤其是与从松节油诱导局部炎症的大鼠分离出的肝脏相似。
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本文引用的文献
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Lack of secretion of serum protein by transplanted rat hepatomas.
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Studies on acute phase proteins of rat serum. 3. Site of synthesis of albumin and alpha 1-acid glycoprotein and the contents of these proteins in liver microsome fractions from rats suffering from induced inflammation.大鼠血清急性期蛋白的研究。3. 白蛋白和α1-酸性糖蛋白的合成部位以及诱导炎症大鼠肝脏微粒体组分中这些蛋白质的含量。
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Biochemistry. 1970 Jul 21;9(15):3059-62. doi: 10.1021/bi00817a019.
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