莫里斯肝癌切片与大鼠肝脏合成及分泌血浆白蛋白、纤维蛋白原和α2-巨球蛋白的比较
Comparison of synthesis and secretion of plasma albumin, fibrinogen and alpha 2-macroglobulin by slices of Morris hepatomas and rat liver.
作者信息
Koj A
出版信息
Br J Exp Pathol. 1980 Jun;61(3):332-8.
Abstract
Slices of rat liver or Morris Hepatomas 5123 GVI and 7777 were incubated for 2 h with 14C-leucine. Newly synthesized labelled proteins were determined independently in the medium and tissue homogenate, total protein after precipitation with trichloro-acetic acid, and albumin, fibrinogen and alpha 2-macroglobulin after immuno-precipitation with monospecific antisera. In comparison with control rat liver, synthesis and secretion of albumin were reduced four- to five-fold in the 2 heptomas examined. On the other hand, synthesis and secretio of fibrinogen and alpha 2-macroglobulin in Hepatomas 5123 GVI annd 7777 were similar to rat liver, especially to the liver isolated from rats with turpentine-induced local inflammation.
摘要
将大鼠肝脏切片或莫里斯肝癌5123 GVI和7777与14C-亮氨酸一起孵育2小时。分别在培养基和组织匀浆中测定新合成的标记蛋白质，用三氯乙酸沉淀后的总蛋白质，以及用单特异性抗血清免疫沉淀后的白蛋白、纤维蛋白原和α2-巨球蛋白。与对照大鼠肝脏相比，在所检测的两种肝癌中，白蛋白的合成和分泌减少了四至五倍。另一方面，肝癌5123 GVI和7777中纤维蛋白原和α2-巨球蛋白的合成和分泌与大鼠肝脏相似，尤其是与从松节油诱导局部炎症的大鼠分离出的肝脏相似。
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