Malinda K M, Sidhu G S, Mani H, Banaudha K, Maheshwari R K, Goldstein A L, Kleinman H K
Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD 20892-4370, USA.
J Invest Dermatol. 1999 Sep;113(3):364-8. doi: 10.1046/j.1523-1747.1999.00708.x.
Angiogenesis is an essential step in the repair process that occurs after injury. In this study, we investigated whether the angiogenic thymic peptide thymosin beta4 (Tbeta4) enhanced wound healing in a rat full thickness wound model. Addition of Tbeta4 topically or intraperitoneally increased reepithelialization by 42% over saline controls at 4 d and by as much as 61% at 7 d post-wounding. Treated wounds also contracted at least 11% more than controls by day 7. Increased collagen deposition and angiogenesis were observed in the treated wounds. We also found that Tbeta4 stimulated keratinocyte migration in the Boyden chamber assay. After 4-5 h, migration was stimulated 2-3-fold over migration with medium alone when as little as 10 pg of Tbeta4 was added to the assay. These results suggest that Tbeta4 is a potent wound healing factor with multiple activities that may be useful in the clinic.
血管生成是损伤后修复过程中的一个重要步骤。在本研究中，我们调查了血管生成性胸腺肽胸腺素β4（Tβ4）是否能在大鼠全层伤口模型中促进伤口愈合。在伤口后第4天，局部或腹腔内添加Tβ4使再上皮化比生理盐水对照组增加42%，在第7天增加多达61%。到第7天，处理过的伤口收缩也比对照组至少多11%。在处理过的伤口中观察到胶原沉积增加和血管生成。我们还发现，在博伊登室试验中，Tβ4刺激角质形成细胞迁移。在4-5小时后，当向试验中添加低至10 pg的Tβ4时，迁移比仅用培养基时刺激了2-3倍。这些结果表明，Tβ4是一种具有多种活性的强效伤口愈合因子，可能在临床上有用。
