Zhu Tingting, Park Hee Chul, Son Kyung Mi, Kwon Ji Hyun, Park Jong-Chul, Yang Hyeong-Cheol
Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University, Seoul 110-749, Republic of Korea.
Department of Medical Engineering, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea.
Int J Mol Med. 2014 Sep;34(3):816-21. doi: 10.3892/ijmm.2014.1832. Epub 2014 Jul 2.
The objective of the present study was to investigate the effect of thymosin β4 (Tβ4) on the wound healing of rat palatal (RP) mucosa and related cellular properties. Cell viability, adhesion and migration of primary cultured RP cells were observed in the presence of Tβ4 at various concentrations ranging from 1 to 1,000 ng/ml. The mRNA and protein expression of matrix metalloproteinase 2 (MMP2) and vascular endothelial growth factor (VEGF) in Tβ4-treated RP cells was assessed by quantitative polymerase chain reaction (RT-qPCR) and western blot analysis, respectively. For the in vivo assay, Tβ4 was applied to excisional wounds (3 mm in diameter) that were made in the center of the palate (n=6). Images of the wound areas were captured and assessed histologically one week after surgery. Tβ4 did not affect cell viability and adhesion, but RP cell migration was stimulated by Tβ4 at concentrations of 100 and 1,000 ng/ml. Tβ4 also increased the mRNA and protein expression of MMP2 and VEGF in RP cells. In the animal model, palatal wound closure was significantly enhanced in rats treated with Tβ4. The results of the present study indicated that Tβ4 promotes the wound healing of RP mucosa. Enhancement of RP cell migration and angiogenesis is likely to be involved in the promotion of wound healing.
本研究的目的是探讨胸腺素β4（Tβ4）对大鼠腭黏膜（RP）伤口愈合及相关细胞特性的影响。在1至1000 ng/ml的不同浓度Tβ4存在下，观察原代培养的RP细胞的细胞活力、黏附及迁移情况。分别通过定量聚合酶链反应（RT-qPCR）和蛋白质印迹分析评估Tβ4处理的RP细胞中基质金属蛋白酶2（MMP2）和血管内皮生长因子（VEGF）的mRNA和蛋白表达。对于体内试验，将Tβ4应用于腭部中央制作的直径3 mm的切除伤口（n = 6）。术后一周拍摄伤口区域图像并进行组织学评估。Tβ4不影响细胞活力和黏附，但100和1000 ng/ml浓度的Tβ4可刺激RP细胞迁移。Tβ4还增加了RP细胞中MMP2和VEGF的mRNA和蛋白表达。在动物模型中，Tβ4处理的大鼠腭部伤口闭合明显加快。本研究结果表明，Tβ4促进RP黏膜伤口愈合。RP细胞迁移和血管生成的增强可能参与了伤口愈合的促进过程。
