Tazawa K, Kurihara Y, Kamoshida S, Tsukada K, Tsutsumi Y
Department of Pathology, Tokai University School of Medicine, Bohseidai, Isehara, Japan.
Pathol Int. 1999 Jun;49(6):500-5. doi: 10.1046/j.1440-1827.1999.00900.x.
Immunoreactivity of prostate-specific antigen (PSA), a kallikrein-like enzyme present in the seminal plasma, was demonstrated by indirect immunoperoxidase staining using a PSA antiserum in the apical cytoplasm along the luminal border of small-sized duct epithelial cells of the major salivary (parotid and submandibular) gland of both sexes (56/56, 100%). No PSA-like immunoreactivity was seen in large-sized duct epithelial cells and acinar cells. Minor salivary gland ducts were negative. When inflammatory and atrophic changes were observed, ductal expression of PSA-like immunoreactivity was decreased (12/37, 32%) and the site of intracellular localization often became diffusely cytoplasmic. The immunoreactivity was absorbed by human seminal plasma. Immunoreactivities of prostatic acid phosphatase and sex hormone receptors were undetectable in the salivary gland. Twenty-nine (34%) of 86 salivary gland tumors with ductal differentiation were immunoreactive for PSA mainly in the cytoplasm. A PSA monoclonal antibody ER-PR8 detected immunoreactivity in the prostate but not in the salivary glands or their tumors. Prostate-specific antigen-like immunoreactivity in small-sized (intercalated) duct epithelial cells of the major salivary gland and their tumors may be due to cross-reactivity of the antiserum with kallikrein-like substances.
前列腺特异性抗原（PSA）是一种存在于精浆中的激肽释放酶样酶，利用PSA抗血清通过间接免疫过氧化物酶染色在两性主要唾液腺（腮腺和颌下腺）小导管上皮细胞管腔缘的顶端细胞质中显示出免疫反应性（56/56，100%）。在大导管上皮细胞和腺泡细胞中未见PSA样免疫反应性。小唾液腺导管呈阴性。当观察到炎症和萎缩性改变时，PSA样免疫反应性的导管表达降低（12/37，32%），细胞内定位部位常变为弥漫性细胞质。该免疫反应性可被人类精浆吸收。在唾液腺中未检测到前列腺酸性磷酸酶和性激素受体的免疫反应性。86例具有导管分化的唾液腺肿瘤中有29例（34%）对PSA呈免疫反应性，主要在细胞质中。PSA单克隆抗体ER-PR8在前列腺中检测到免疫反应性，但在唾液腺及其肿瘤中未检测到。主要唾液腺及其肿瘤的小（闰）导管上皮细胞中的前列腺特异性抗原样免疫反应性可能是由于抗血清与激肽释放酶样物质的交叉反应所致。
