Klentzeris L D, Bulmer J N, Warren M A, Morrison L, Li T C, Cooke I D
University Department of Obstetrics and Gynaecology, Jessop Hospital for Women, Sheffield, UK.
Hum Reprod. 1994 Apr;9(4):646-52. doi: 10.1093/oxfordjournals.humrep.a138564.
The purpose of this study was to investigate whether the endometrium of women with unexplained infertility differs in some immunological aspects from the endometrium of normal fertile women. Endometrial biopsies were obtained from 24 normal fertile women (group I) and 24 women suffering from unexplained infertility (group II) at 4, 7, 10 and 13 days following the luteinizing hormone (LH) surge. Endometrial granulated lymphocytes were assessed morphometrically in 2 microns resin sections. A panel of 11 monoclonal antibodies was employed to characterize the leukocyte subsets in frozen sections. Semi-quantification was performed with a Quantimet 970 image analyser. Data were analysed using one- and two-way analysis of variance. Compared with fertile controls, women with unexplained infertility had significantly lower numbers of CD8+ (T suppressor/cytotoxic) cells at each post-LH date. In contrast, the number of CD4+ (T helper/inducer) cells was significantly higher in group II. Throughout the luteal phase, infertile women had fewer CD56+ cells than normal fertile controls. The volume fraction of endometrium occupied by the nuclei of endometrial granulated lymphocytes did not alter with the cycle stage but the mean nuclear diameter and axial ratio decreased from LH+7 to LH+13. The differences observed in endometrial leukocytic subpopulations between fertile and infertile women may contribute to unexplained infertility probably by affecting the embryonic maternal dialogue during the implantation and early placentation period.
本研究的目的是调查不明原因不孕症女性的子宫内膜在某些免疫学方面是否与正常可育女性的子宫内膜存在差异。在促黄体生成素（LH）峰后的第4、7、10和13天，从24名正常可育女性（I组）和24名患有不明原因不孕症的女性（II组）获取子宫内膜活检样本。在2微米的树脂切片中对子宫内膜颗粒淋巴细胞进行形态计量学评估。使用一组11种单克隆抗体对冰冻切片中的白细胞亚群进行表征。使用Quantimet 970图像分析仪进行半定量分析。数据采用单因素和双因素方差分析。与可育对照组相比，不明原因不孕症女性在LH峰后的每个日期CD8 +（T抑制/细胞毒性）细胞数量均显著降低。相反，II组中CD4 +（T辅助/诱导）细胞数量显著更高。在整个黄体期，不孕女性的CD56 +细胞比正常可育对照组少。子宫内膜颗粒淋巴细胞细胞核所占子宫内膜的体积分数不随月经周期阶段而改变，但平均核直径和轴比从LH + 7到LH + 13逐渐减小。可育和不孕女性子宫内膜白细胞亚群的差异可能通过影响着床和早期胎盘形成期的胚胎与母体对话而导致不明原因不孕症。
