Maruyama M, Asano T, Nakagohri T, Uematsu T, Hasegawa M, Miyauchi H, Iwashita C, Isono K
Second Department of Surgery, Chiba University School of Medicine, Japan.
Anticancer Res. 1999 May-Jun;19(3A):1989-93.
The anti-tumor effects of high-energy shock waves (HESW) in combination with cisplatin (CDDP) and ATX-70, as a photosensitizer, were investigated in vitro and in vivo.
Active oxygen generation by HESW was studied by means of electron spin resonance (ESR) system. The anti-tumor effects of HESW in combination with cisplatin (CDDP) and ATX-70 was investigated in vitro and in vivo.
HESW exposure induced singlet oxygen or hydroxyl radical. Adding ATX-70 enhanced the cell damage induced by HESW dose-dependently. Ascorbic acid inhibited the combined effect of HESW and ATX-70. Adding CDDP did not enhanced cell damage by HESW. The combined therapy of HESW, CDDP and ATX-70 showed further inhibition of tumor growth in vivo.
These results indicate that HESW activated ATX-70, and that the anti-tumor effect of HESW and ATX-70 was caused by active oxygen generation. HESW is able to be utilized with any other cancer treatment.
研究了高能冲击波（HESW）联合顺铂（CDDP）和作为光敏剂的ATX-70在体外和体内的抗肿瘤作用。
通过电子自旋共振（ESR）系统研究HESW产生活性氧的情况。在体外和体内研究了HESW联合顺铂（CDDP）和ATX-70的抗肿瘤作用。
HESW照射可诱导单线态氧或羟基自由基。添加ATX-70剂量依赖性地增强了HESW诱导的细胞损伤。抗坏血酸抑制了HESW和ATX-70的联合作用。添加CDDP并未增强HESW对细胞的损伤。HESW、CDDP和ATX-70的联合治疗在体内显示出对肿瘤生长的进一步抑制作用。
这些结果表明HESW激活了ATX-70，且HESW和ATX-70的抗肿瘤作用是由活性氧的产生引起的。HESW能够与任何其他癌症治疗方法联合使用。
