Canaparo Roberto, Serpe Loredana, Catalano Maria Graziella, Bosco Ornella, Zara Gian Paolo, Berta Laura, Frairia Roberto
Department of Clinical Pathophysiology, University of Torino, Via Genova 3, 10126 Torino, Italy.
Anticancer Res. 2006 Sep-Oct;26(5A):3337-42.
Whether high energy shock waves (HESW), generated by a piezoelectric generator, were able to activate a sonosensitizer, 5-aminolevulinic acid (ALA) and induce inhibition of cell growth in HT-29 human colorectal cancer cells was investigated.
Cell survival and cell death pathways were investigated by cell growth curves, flow cytometry analysis and ELISA nucleosome evaluation. HT-29 cells were exposed to ALA and different HESW treatments: E1 (energy flux density = 0.22 mJ/mm2; 500 and 1000 shots) and E2 (energy flux density = 0.88 mJ/mm2; 500 and 1000 shots).
A significant reduction of HT-29 cell growth with respect to untreated cells was observed only after treatment with ALA and HESW E2, 500 shots. In particular, HESWE2, 500 shots, was able to induce an arrest of HT-29 cells exposed to ALA in the G0/G1-phase of the cell cycle.
HESW is proposed for the sonodynamic treatment of cancer cells.
研究了由压电发生器产生的高能冲击波（HESW）是否能够激活声敏剂5-氨基乙酰丙酸（ALA）并诱导HT-29人结肠癌细胞的生长抑制。
通过细胞生长曲线、流式细胞术分析和ELISA核小体评估研究细胞存活和细胞死亡途径。将HT-29细胞暴露于ALA和不同的HESW处理：E1（能量通量密度=0.22 mJ/mm2；500次和1000次冲击）和E2（能量通量密度=0.88 mJ/mm2；500次和1000次冲击）。
仅在用ALA和HESW E2处理500次后，观察到HT-29细胞生长相对于未处理细胞有显著降低。特别是，HESW E2处理500次能够诱导暴露于ALA的HT-29细胞在细胞周期的G0/G1期停滞。
提出将HESW用于癌细胞的声动力治疗。
