König B, Oed M, Kunz A, Schlett R, Mack M
Anticancer Res. 1999 Jul-Aug;19(4A):2739-41.
An immunoassay utilizing chemiluminescence and paramagnetic particles has been developed for the new fully automated random access LIAISON immunoanalyzer. The LIAISON Ferritin assay is a two-site immunoluminometric one-step assay using two highly specific monoclonal antibodies. A specially designed unique Reagent Integral contains all specific reagents. The cooled reagent compartment guarantees an on-board stability of at least 4 weeks. The assay works with a 2-point calibrated master curve. To perform, the assay, 10 microliters sample is added to 300 microliters tracer and 20 microliters antibody-coated magnetic particles. After 10 min incubation the particles are separated, washed and the chemiluminescent signal is generated. The time to first result is only 15 min. The assay with a unique extended standard range up to 3,000 ng/ml shows no high dose hook effect up to 150,000 ng/ml (spiked sera). The cross-reactivity to human liver ferritin is 100%, to human spleen ferritin 66% and to human heart ferritin 8%. Precision (within-run < 3%; between-run < 5%), linearity, recovery and sensitivity (< 0.2 ng/ml) are excellent. The assay shows a very good correlation to LlA-mat Ferritin (r = 0.994). In summary the LIAISON Ferritin run on the new LIAISON immunoanalyzer and is a very rapid and accurate method rapidly providing reliable results.
已为新型全自动随机存取LIAISON免疫分析仪开发了一种利用化学发光和顺磁性颗粒的免疫测定法。LIAISON铁蛋白测定法是一种双位点免疫发光一步测定法，使用两种高度特异性的单克隆抗体。一个经过特殊设计的独特试剂整合包包含所有特定试剂。冷却的试剂隔层可确保至少4周的机载稳定性。该测定法采用两点校准的主曲线。进行该测定时，将10微升样品加入300微升示踪剂和20微升抗体包被的磁性颗粒中。孵育10分钟后，分离颗粒、洗涤并产生化学发光信号。首次出结果的时间仅为15分钟。该测定法具有高达3000 ng/ml的独特扩展标准范围，在高达150,000 ng/ml（加标血清）时未显示高剂量钩效应。与人肝铁蛋白的交叉反应性为百分之百，与人脾铁蛋白的交叉反应性为66%，与人心脏铁蛋白的交叉反应性为8%。精密度（批内<3%；批间<5%）、线性、回收率和灵敏度（<0.2 ng/ml）都非常出色。该测定法与LIA-mat铁蛋白显示出非常好的相关性（r = 0.994）。总之，在新型LIAISON免疫分析仪上运行的LIAISON铁蛋白测定法是一种非常快速准确的方法，能迅速提供可靠结果。
