Dupuy Anne Marie, Debarge Laurent, Poulain Marine, Badiou Stéphanie, Rossi Michel, Cristol Jean Paul
Department of Biochemistry, Lapeyronie Hospital, Montpellier, France.
Clin Lab. 2009;55(5-6):207-15.
The implementation of a ferritin assay other that by the RIA technique may be necessary in several laboratories. Indeed the RIA method presents limitations for routine determination: use of radioactive isotopes, high cost of reagents and low capacity (manual technique). This method was not suitable for all laboratories.
The aim of this study was to compare the IRMA method with non-isotope methods (immunoturbidimetric assay and chemiluminescence detection) for determination of ferritin in serum samples.
Analytical performances of Olympus-, Horiba ABX- and Beckman Coulter- ferritin are good. In a whole population (n = 124) linear regression analysis indicated excellent agreement between ferritin values obtained on all patients leading to a coefficient of determination r2 > 0.96, 0.95 and 0.96 with Olympus-Ferritin, Horiba ABX-ferritin, and Beckman-ferrritin, respectively. Results of Bland-Altman analysis demonstrated the excellent concordance of ferritin methods with samples.
Immunoturbidimetric methods, particularly the Horiba ABX method on Pentra 400 and immunoenzymometric assay with chemiluminescence detection with Beckman Coulter on Access 2, are a reasonable alternative to the established RIA for all laboratories.
在一些实验室中，采用除放射免疫分析（RIA）技术之外的铁蛋白检测方法可能是必要的。实际上，RIA方法在常规检测中存在局限性：使用放射性同位素、试剂成本高以及检测能力低（手工技术）。这种方法并不适用于所有实验室。
本研究的目的是比较免疫放射分析（IRMA）方法与非同位素方法（免疫比浊法和化学发光检测法）在血清样本中铁蛋白测定方面的差异。
奥林巴斯、堀场ABX和贝克曼库尔特铁蛋白检测的分析性能良好。在全体人群（n = 124）中，线性回归分析表明，所有患者的铁蛋白值之间具有极好的一致性，奥林巴斯铁蛋白、堀场ABX铁蛋白和贝克曼铁蛋白的决定系数r2分别> 0.96、0.95和0.96。布兰德-奥特曼分析结果表明铁蛋白检测方法与样本具有极好的一致性。
免疫比浊法，特别是堀场ABX在Pentra 400上的检测方法以及贝克曼库尔特在Access 2上的化学发光免疫酶测定法，对于所有实验室而言，都是已确立的RIA方法的合理替代方法。
