Oztezcan S, Türkoğlu U M, Kervancioğlu E, Koçak T, Koçak-Toker N, Aykaç-Toker G
Department of Biochemistry and Urology, Istanbul University, Istanbul Faculty of Medicine, Turkey.
Andrologia. 1999 Jul;31(4):195-8. doi: 10.1046/j.1439-0272.1999.00279.x.
In the present study, the in vitro effects of peroxynitrite on sperm motility, lipid peroxidation and sulfhydryl content were examined. Sperm percentage motility and movement characteristics were assessed by a computer-assisted system. Lipid peroxidation was measured by determining malondialdehyde levels using the thiobarbituric acid (TBA) method. Sperm sulfhydryl content was measured by a spectrophotometric method based on reduction of 5,5'-dithiobis-(2-nitrobenzoic acid) by sulfhydryl groups. Percentage motility, movement characteristics and sulfhydryl content decreased significantly in peroxynitrite-treated samples compared to decomposed peroxynitrite-treated samples. Lipid peroxidation in peroxynitrite-treated samples was significantly higher than in decomposed peroxynitrite-treated samples. These results indicate that peroxynitrite anion may cause sperm dysfunction through lipid peroxidation stimulation and total SH depletion.
在本研究中，检测了过氧亚硝酸盐对精子活力、脂质过氧化和巯基含量的体外作用。精子活力百分比和运动特征通过计算机辅助系统进行评估。脂质过氧化通过硫代巴比妥酸（TBA）法测定丙二醛水平来衡量。精子巯基含量通过基于巯基还原5,5'-二硫代双（2-硝基苯甲酸）的分光光度法进行测定。与经分解过氧亚硝酸盐处理的样品相比，经受过氧亚硝酸盐处理的样品的活力百分比、运动特征和巯基含量显著降低。经受过氧亚硝酸盐处理的样品中的脂质过氧化显著高于经分解过氧亚硝酸盐处理的样品。这些结果表明，过氧亚硝酸盐阴离子可能通过刺激脂质过氧化和耗尽总巯基导致精子功能障碍。
