Biswas T, Roy S, Banerjee K K
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.
Immunology. 1995 Feb;84(2):322-5.
The induction of proliferation of murine splenocytes by lipopolysaccharide (LPS) of Shigella dysenteriae type 1 and its polysaccharide (PS) and lipid A fractions was investigated. The LPS-induced proliferation reached a maximum at a concentration of 30 ng/ml. The PS and lipid A induced proliferation of murine splenocytes at similar concentrations. Preincubation of murine splenocytes with varying concentrations of L-rhamnose blocked LPS- and PS-induced proliferation in a dose-dependent manner. The lipid A-induced stimulation, on the contrary, was not affected by preincubation of the cells with L-rhamnose. These data suggest that activation of splenocytes by LPS and PS is mechanistically different from that induced by lipid A and is presumably involved in the specific recognition of carbohydrate structures on LPS and PS.
研究了痢疾志贺氏菌1型脂多糖（LPS）及其多糖（PS）和脂质A组分对小鼠脾细胞增殖的诱导作用。LPS诱导的增殖在浓度为30 ng/ml时达到最大值。PS和脂质A在相似浓度下诱导小鼠脾细胞增殖。用不同浓度的L-鼠李糖预孵育小鼠脾细胞，以剂量依赖的方式阻断LPS和PS诱导的增殖。相反，脂质A诱导的刺激不受细胞与L-鼠李糖预孵育的影响。这些数据表明，LPS和PS对脾细胞的激活在机制上与脂质A诱导的不同，可能涉及对LPS和PS上碳水化合物结构的特异性识别。
