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深红红螺菌固氮酶的纯化及性质，以及与铁蛋白共价结合的磷酸盐、核糖和腺嘌呤样单元的证据。

Purification and properties of nitrogenase from Rhodospirillum rubrum, and evidence for phosphate, ribose and an adenine-like unit covalently bound to the iron protein.

作者信息

Ludden P W, Burris R H

出版信息

Biochem J. 1978 Oct 1;175(1):251-9. doi: 10.1042/bj1750251.

DOI:10.1042/bj1750251
PMID:104713
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1186061/
Abstract
  1. The molybdenum-iron (Mo-Fe) protein, iron (Fe) protein and the activating factor of nitrogenase from Rhodospirillum rubrum were purified. 2. The Mo-Fe protein has properties similar to those of the Mo-Fe proteins of other nitrogen-fixing organisms. 3. The Fe protein is similar to other Fe proteins with respect to its molecular weight, metal composition and e.p.r. signal. 4. The Fe protein is different from other Fe proteins in that it apparently has two types of subunits rather than one, its u.v. spectrum has an extra peak, and phosphate, ribose and an adenine-like unit are covalently bound to the protein. The presence of these non-protein groups on the protein may explain the requirement for activation of R. rubrum Fe protein.
摘要
  1. 对深红红螺菌的钼铁（Mo-Fe）蛋白、铁（Fe）蛋白和固氮酶激活因子进行了纯化。2. 该Mo-Fe蛋白具有与其他固氮生物的Mo-Fe蛋白相似的性质。3. 就分子量、金属组成和电子顺磁共振信号而言，该Fe蛋白与其他Fe蛋白相似。4. 该Fe蛋白与其他Fe蛋白的不同之处在于，它显然有两种亚基而非一种，其紫外光谱有一个额外的峰，并且磷酸盐、核糖和一个类腺嘌呤单元与该蛋白共价结合。蛋白上这些非蛋白基团的存在可能解释了深红红螺菌Fe蛋白激活的需求。
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d978/1186061/7f19c8cfa718/biochemj00477-0254-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d978/1186061/acca0d9dd5d0/biochemj00477-0253-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d978/1186061/7f19c8cfa718/biochemj00477-0254-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d978/1186061/acca0d9dd5d0/biochemj00477-0253-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d978/1186061/7f19c8cfa718/biochemj00477-0254-a.jpg

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Purification and properties of nitrogenase from Rhodospirillum rubrum, and evidence for phosphate, ribose and an adenine-like unit covalently bound to the iron protein.深红红螺菌固氮酶的纯化及性质，以及与铁蛋白共价结合的磷酸盐、核糖和腺嘌呤样单元的证据。
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Purification of P(II) and P(II)-UMP and in vitro studies of regulation of glutamine synthetase in Rhodospirillum rubrum.红螺菌中P(II)和P(II)-UMP的纯化以及谷氨酰胺合成酶调控的体外研究。
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NAD-dependent cross-linking of dinitrogenase reductase and dinitrogenase reductase ADP-ribosyltransferase from Rhodospirillum rubrum.来自红螺菌的二氮酶还原酶与二氮酶还原酶 ADP 核糖基转移酶的 NAD 依赖性交联。
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Characterization of the CO-induced, CO-tolerant hydrogenase from Rhodospirillum rubrum and the gene encoding the large subunit of the enzyme.来自红螺菌的一氧化碳诱导型、一氧化碳耐受型氢化酶及其编码该酶大亚基的基因的表征。
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A micro biuret method for protein determination; determination of total protein in cerebrospinal fluid.一种用于蛋白质测定的微量双缩脲法；脑脊液中总蛋白的测定
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