腺嘌呤掺入到深红红螺菌固氮酶无活性铁蛋白的修饰基团中。
Incorporation of adenine into the modifying group of inactive iron protein of nitrogenase from Rhodospirillum rubrum.
作者信息
Nordlund S, Ludden P W
出版信息
Biochem J. 1983 Mar 1;209(3):881-4. doi: 10.1042/bj2090881.
Abstract
Adenine was fed to cells of Rhodospirillum rubrum grown on glutamate. The adenine was found to be incorporated into the modifying group of the inactive form of iron protein. Adenine labelled in the 8-position ([8-3H]adenine) and the 2-position ([2-3H]adenine) was specifically incorporated into the electrophoretic 'upper-band' subunit of iron protein. Incorporation of label from the 2-position into many proteins was observed if histidine was not present in the medium. Label was removed by the activating enzyme for iron protein.
摘要
将腺嘌呤添加到在谷氨酸上生长的深红红螺菌细胞中。发现腺嘌呤被掺入到铁蛋白无活性形式的修饰基团中。在8位（[8-³H]腺嘌呤）和2位（[2-³H]腺嘌呤）标记的腺嘌呤被特异性地掺入到铁蛋白的电泳“上带”亚基中。如果培养基中不存在组氨酸，则观察到2位的标记掺入到许多蛋白质中。标记被铁蛋白的激活酶去除。
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