De Azavedo J C, Yeung R H, Bast D J, Duncan C L, Borgia S B, Low D E
Department of Microbiology, Mount Sinai and Princess Margaret Hospitals, University of Toronto, Toronto, Ontario, Canada.
Antimicrob Agents Chemother. 1999 Sep;43(9):2144-7. doi: 10.1128/AAC.43.9.2144.
A total of 3,205 group A streptoccal isolates were collected in 1997 through a private laboratory which serves community physicians in southern Ontario and which represents a population base of 6 million people. Nonsusceptibility to erythromycin was detected for 67 (2.1%) isolates both by disk diffusion and by broth microdilution. Of these, 47 (70%) were susceptible to clindamycin and were found by PCR to possess the mef gene. Of the other 20 strains, 18 and 2 showed inducible and constitutive resistance, respectively, to clindamycin. Nineteen of these strains were shown by PCR to possess the ermTR gene, and a single constitutively resistant strain harbored an ermB gene. Sixteen (24%) erythromycin-resistant strains were also resistant to tetracycline. All were susceptible to penicillin and chloramphenicol.
1997年,通过一家为安大略省南部社区医生服务的私人实验室收集了总共3205株A组链球菌分离株,该实验室代表了600万人口的基数。通过纸片扩散法和肉汤微量稀释法检测到67株(2.1%)分离株对红霉素不敏感。其中,47株(70%)对克林霉素敏感,经聚合酶链反应(PCR)检测发现含有mef基因。在另外20株菌株中,分别有18株和2株对克林霉素表现出诱导型和组成型耐药。经PCR检测,其中19株菌株含有ermTR基因,1株组成型耐药菌株含有ermB基因。16株(24%)耐红霉素菌株也对四环素耐药。所有菌株对青霉素和氯霉素均敏感。