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Evaluation of different approaches for fetal DNA analysis from maternal plasma and nucleated blood cells.

作者信息

Smid M, Lagona F, de Benassuti L, Ferrari A, Ferrari M, Cremonesi L

机构信息

Istituto di Rivocero e Cura a Carattere Scientifico, Hospital San Raffaele, Department of Obstetrics and Gynecology, Via Olgettina 60, 20132 Milan, Italy.

出版信息

Clin Chem. 1999 Sep;45(9):1570-2.

PMID:10471665
Abstract
摘要

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Evaluation of different approaches for fetal DNA analysis from maternal plasma and nucleated blood cells.评估从孕妇血浆和有核血细胞中进行胎儿DNA分析的不同方法。
Clin Chem. 1999 Sep;45(9):1570-2.
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The quest for accurate measurement of fetal DNA in maternal plasma.在母体血浆中精确测量胎儿DNA的探索。
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Improved fetal nucleated erythrocyte sorting purity using intracellular antifetal hemoglobin and Hoechst 33342.使用细胞内抗胎儿血红蛋白和Hoechst 33342提高胎儿有核红细胞分选纯度。
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Limited feasibility of routinely analyzing fetal cells from maternal blood by using magnetic activated cell sorting and polymerase chain reaction for prenatal diagnosis.利用磁珠分选和聚合酶链反应从母体血液中常规分析胎儿细胞用于产前诊断的可行性有限。
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Y-chromosome DNA is present in the blood of female dogs suggesting the presence of fetal microchimerism.Y 染色体 DNA 存在于雌性犬的血液中,提示存在胎儿微嵌合体。
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