Tichý P, Rytír V, Sobotková E, Fuchsová Z
Folia Microbiol (Praha). 1976;21(2):100-6. doi: 10.1007/BF02876976.
Glucosamine added to a transformation medium (TM2) after a 30-min cultivation of cells exhibited the highest inhibitory effect on the transformation process in Bacillus subtilis 168 trp2. The recipient culture was least sensitive to glucosamine added after 50 min. Glucosamine had no inhibitory effect when added 10 min after the transformation DNA.
在细胞培养30分钟后添加到转化培养基(TM2)中的葡萄糖胺对枯草芽孢杆菌168 trp2的转化过程表现出最高的抑制作用。受体培养物对50分钟后添加的葡萄糖胺最不敏感。在转化DNA 10分钟后添加葡萄糖胺没有抑制作用。