作为突触小泡内吞作用钙传感器的钙调神经磷酸酶-发动蛋白1复合物
The calcineurin-dynamin 1 complex as a calcium sensor for synaptic vesicle endocytosis.
作者信息
Lai M M, Hong J J, Ruggiero A M, Burnett P E, Slepnev V I, De Camilli P, Snyder S H
机构信息
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
出版信息
J Biol Chem. 1999 Sep 10;274(37):25963-6. doi: 10.1074/jbc.274.37.25963.
Exocytosis of synaptic vesicles is calcium-dependent, with synaptotagmin serving as the calcium sensor. Endocytosis of synaptic vesicles has also been postulated as a calcium-dependent process; however, an endocytic calcium sensor has not been found. We now report a physical association between the calcium-dependent phosphatase calcineurin and dynamin 1, a component of the synaptic endocytic machinery. The calcineurin-dynamin 1 interaction is calcium-dependent, with an EC(50) for calcium in the range of 0.1-0. 4 microM. Disruption of the calcineurin-dynamin 1 interaction inhibits clathrin-mediated endocytosis. Thus, the calcium-dependent formation of the calcineurin-dynamin 1 complex, delivered to the other endocytic coat proteins, provides a calcium-sensing mechanism that facilitates endocytosis.
突触小泡的胞吐作用依赖于钙，突触结合蛋白作为钙传感器。突触小泡的内吞作用也被假定为一个依赖于钙的过程；然而，尚未发现内吞钙传感器。我们现在报告钙依赖性磷酸酶钙调神经磷酸酶与发动蛋白1之间的物理关联，发动蛋白1是突触内吞机制的一个组成部分。钙调神经磷酸酶与发动蛋白1的相互作用依赖于钙，钙的半数有效浓度（EC50）在0.1 - 0.4微摩尔范围内。钙调神经磷酸酶与发动蛋白1相互作用的破坏会抑制网格蛋白介导的内吞作用。因此，钙调神经磷酸酶与发动蛋白1复合物的钙依赖性形成，传递给其他内吞衣被蛋白，提供了一种促进内吞作用的钙传感机制。
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