突触素调节突触小泡的网格蛋白非依赖性内吞作用。
Synaptophysin regulates clathrin-independent endocytosis of synaptic vesicles.
作者信息
Daly C, Sugimori M, Moreira J E, Ziff E B, Llinás R
机构信息
Howard Hughes Medical Institute and Department of Biochemistry, New York University Medical Center, New York, NY 10016, USA.
出版信息
Proc Natl Acad Sci U S A. 2000 May 23;97(11):6120-5. doi: 10.1073/pnas.97.11.6120.
Abstract
The GTPase dynamin I is required for synaptic vesicle (SV) endocytosis. Our observation that dynamin binds to the SV protein synaptophysin in a Ca(2+)-dependent fashion suggested the possibility that a dynamin/synaptophysin complex functions in SV recycling. In this paper we show that disruption of the dynamin/synaptophysin interaction by peptide injection into the squid giant synapse preterminal results in a decrease in transmitter release during high-frequency stimulation, indicating an inhibition of SV recycling. Electron microscopy of these synapses reveals a depletion of SVs, demonstrating a block of vesicle retrieval after fusion. In addition, we observed an increase in clathrin-coated vesicles, indicating that the peptide does not block clathrin-dependent endocytosis. We conclude that the dynamin/synaptophysin complex functions in a clathrin-independent mechanism of SV endocytosis that is required for efficient synaptic transmission.
摘要
GTP酶动力蛋白I是突触小泡（SV）内吞作用所必需的。我们观察到动力蛋白以Ca(2+)依赖的方式与SV蛋白突触素结合，这提示了动力蛋白/突触素复合物在SV循环中发挥作用的可能性。在本文中，我们表明，通过向鱿鱼巨大突触终末前注射肽来破坏动力蛋白/突触素的相互作用，会导致高频刺激期间递质释放减少，这表明SV循环受到抑制。对这些突触的电子显微镜观察显示SV耗竭，表明融合后囊泡回收受阻。此外，我们观察到网格蛋白包被囊泡增加，这表明该肽不会阻断网格蛋白依赖的内吞作用。我们得出结论，动力蛋白/突触素复合物在一种不依赖网格蛋白的SV内吞机制中发挥作用，而这种机制是高效突触传递所必需的。
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