Chung J, Park Y, Paek S, Chong Y, Kim W
Department of Ophthalmology, Medical College, Ewha Women's University, Seoul, Korea.
Ophthalmic Res. 1999;31(6):432-9. doi: 10.1159/000055568.
The healing of stroma and endothelium after a standardized corneal alkali wound was evaluated in sodium-hyaluronan (Na-HA)-treated eyes. Alkali wounds were produced in one eye of each rabbit by applying a 5.5-mm round filter paper soaked in 1 N NaOH onto the central cornea for 60 s. Eyes were then treated with either 1% Na-HA (the treatment group) or phosphate-buffered saline (the control group) 4 times per day for 3 weeks. Endothelial wound morphometry was performed after alizarin red and trypan blue staining, while stromal healing was assessed by counting polymorphonuclear leukocytes (PMNs) and keratocytes in the central and marginal wound areas. During the early healing period the stroma treated with Na-HA had less PMNs than that of the control group. The size of the endothelial defect area measured 5 days after injury was significantly smaller in the Na-HA group than in the control corneas. The present findings indicate that topically applied 1% Na-HA affects stromal and endothelial healing during the early repair process after corneal alkali wounds.
在接受透明质酸钠（Na - HA）治疗的眼睛中，评估了标准化角膜碱烧伤后基质和内皮的愈合情况。通过将浸泡在1N氢氧化钠中的5.5毫米圆形滤纸敷于每只兔子的一只眼睛的中央角膜60秒，造成碱烧伤。然后，每天用1% Na - HA（治疗组）或磷酸盐缓冲盐水（对照组）治疗眼睛4次，持续3周。在茜素红和台盼蓝染色后进行内皮伤口形态测量，而通过计数中央和边缘伤口区域的多形核白细胞（PMN）和角膜细胞来评估基质愈合情况。在早期愈合阶段，用Na - HA治疗的基质中的PMN比对照组少。损伤后5天测量的内皮缺损区域大小，Na - HA组明显小于对照角膜。目前的研究结果表明，局部应用1% Na - HA会影响角膜碱烧伤后早期修复过程中的基质和内皮愈合。
