Chung J H, Kim W K, Lee J S, Pae Y S, Kim H J
Department of Ophthalmology, College of Medicine, Ewha Women's University, Seoul, Korea.
Ophthalmic Res. 1998;30(2):96-100. doi: 10.1159/000055460.
The present study investigated the effect of Na-hyaluronan (Na-HA) on the hemidesmosome morphogenesis in n-heptanol-induced corneal wounds. Central epithelial wounds were induced in the rabbit cornea by applying a 5.5-mm round filter paper, which was soaked in n-heptanol, for 60 s. 1% Na-HA in phosphate-buffered saline (PBS) or PBS alone were topically administered to the wounded cornea 4 times daily for up to 7 days. Epithelial healing rates during the first 2 days were not altered by Na-HA. The number of hemidesmosomes in the basement membrane of the central cornea was significantly increased in both 3- and 7-day groups after treatment with 1% Na-HA. The results suggest that topically applied 1% Na-HA may enhance the formation of hemidesmosomes during the early healing phase in n-heptanol-induced corneal wounds.
本研究调查了透明质酸钠（Na-HA）对正庚醇诱导的角膜伤口中半桥粒形态发生的影响。通过应用一张浸泡在正庚醇中的5.5毫米圆形滤纸60秒，在兔角膜中诱导中央上皮伤口。将磷酸盐缓冲盐水（PBS）中的1% Na-HA或单独的PBS每天局部应用于受伤角膜4次，持续7天。Na-HA未改变前两天的上皮愈合率。用1% Na-HA处理后，3天和7天组中央角膜基底膜中的半桥粒数量均显著增加。结果表明，局部应用1% Na-HA可能会在正庚醇诱导角膜伤口的早期愈合阶段增强半桥粒的形成。
