Colvin J S, Feldman B, Nadeau J H, Goldfarb M, Ornitz D M
Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Dev Dyn. 1999 Sep;216(1):72-88. doi: 10.1002/(SICI)1097-0177(199909)216:1<72::AID-DVDY9>3.0.CO;2-9.
Fibroblast growth factor 9 (FGF9), originally cloned as glial-activating factor from human glioma cells, is expressed in adult rat brain and kidney. Here we report the chromosomal localization, genomic organization, and embryonic expression pattern of the mouse Fgf9 gene. Fgf9 maps to chromosome 14 near the Ctla6 locus. The gene spans more than 34 kb and contains three exons and two introns. Translation initiation occurs in exon 1, and translation termination occurs in exon 3. Fgf9 RNA was detected during mouse embryogenesis in several tissues in which Fgf gene expression has not been previously described, including intermediate mesoderm of late-stage gastrulation, ventricular myocardium, lung pleura, skeletal myoblasts in the early limb bud, spinal cord motor neurons, olfactory bulb, and gut lumenal epithelium. Fgf9 is coexpressed with other Fgf genes in some skeletal myoblasts, in limb apical ectoderm, in craniofacial ectoderm, and in the retina, inner ear, and tooth bud. Dev Dyn 1999;216:72-88.
成纤维细胞生长因子9（FGF9）最初是从人胶质瘤细胞中作为神经胶质激活因子克隆出来的，在成年大鼠的脑和肾中表达。在此，我们报告小鼠Fgf9基因的染色体定位、基因组结构及胚胎表达模式。Fgf9定位于14号染色体上靠近Ctla6基因座的位置。该基因跨度超过34kb，包含三个外显子和两个内含子。翻译起始于外显子1，翻译终止于外显子3。在小鼠胚胎发育过程中，在一些此前未描述过Fgf基因表达的组织中检测到了Fgf9 RNA，包括原肠胚晚期的中间中胚层、心室心肌、肺胸膜、早期肢芽中的骨骼肌成肌细胞、脊髓运动神经元、嗅球和肠腔上皮。在一些骨骼肌成肌细胞、肢体顶端外胚层、颅面外胚层以及视网膜、内耳和牙胚中，Fgf9与其他Fgf基因共同表达。《发育动力学》1999年；216：72 - 88。
