Fu Y, Martinez C, Chalar C, Craig P S, Ehrlich R, Petavy A F, Bosquet G
C.G.M.C., UMR CNRS 5534, Université Lyon I, Villeurbanne, France.
Mol Biochem Parasitol. 1999 Jul 30;102(1):43-52. doi: 10.1016/s0166-6851(99)00084-5.
An immunoscreening of a cDNA library derived from the adult stage of the parasitic platyhelminth Echinococcus granulosus has been carried out with sera from infected dogs. The EgA31 clone encodes a fibrous protein which shares some sequence elements with paramyosins. The corresponding gene is present as a single copy in the genome. As revealed by an antibody obtained against a fusion protein produced in bacteria, the polypeptide has a molecular weight of 66 kDa. This polypeptide is present at all developmental stages studied and is a potent antigen during an infection by the adult stage in the dog and during cyst growth in human patients. By immunohistology, it was shown that it is present in the tegument and subtegumental parenchyma of the adult with a main location in the region of the suckers where it rapidly accumulates at the time of the head evagination. It is also present in the germinal layer of the cyst and on the protoscolex.
利用感染犬的血清对源自寄生扁形虫细粒棘球绦虫成虫阶段的cDNA文库进行了免疫筛选。EgA31克隆编码一种纤维蛋白，该蛋白与副肌球蛋白有一些序列元件相同。相应的基因在基因组中以单拷贝形式存在。通过针对细菌中产生的融合蛋白获得的抗体显示，该多肽的分子量为66 kDa。这种多肽存在于所有研究的发育阶段，并且在犬的成虫感染期间以及人类患者囊肿生长期间是一种强效抗原。通过免疫组织学表明，它存在于成虫的皮层和皮层下实质中，主要位于吸盘区域，在头外翻时迅速积累。它也存在于囊肿的生发层和原头节上。
