牛肾上腺嗜铬细胞中高效的Semliki森林病毒介导的转导
High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells.
作者信息
Duncan R R, Don-Wauchope A C, Tapechum S, Shipston M J, Chow R H, Estibeiro P
机构信息
Membrane Biology Group, Biomedical Sciences, University of Edinburgh Medical School, Teviot Place, Edinburgh, EH8 9XD, U.K.
出版信息
Biochem J. 1999 Sep 15;342 Pt 3(Pt 3):497-501.
Abstract
Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki Forest virus (SFV) vectors allow high-efficiency expression of heterologous protein in chromaffin cells.
摘要
肾上腺嗜铬细胞常用于胞吐作用的研究。由于在嗜铬细胞中引入和表达异源cDNA尚无简单高效的技术，分子机制特征研究进展缓慢。在此，我们证明辛德毕斯病毒（SFV）载体可使嗜铬细胞高效表达异源蛋白。
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