Palus J, Dziubałtowska E, Rydzyński K
Nofer Institute of Occupational Medicine, Department of Toxicology and Carcinogenesis, 8 Teresy Strasse, PO Box 199, 90-950, Lódź, Poland.
Mutat Res. 1999 Jul 21;444(1):61-74. doi: 10.1016/s1383-5718(99)00089-3.
The study was aimed at the assessment of genotoxic effects in workers of a wooden furniture manufacture, based on the level of DNA damage in white blood cells (WBC). The alkaline single cell gel electrophoresis assay (known as the comet assay) in individual cells was adapted for detecting damaged DNA in WBC. The level of DNA damage was determined as the percentage of cells with comets. It was assessed in cells before and after incubation in RPMI 1640 medium and CO(2) at 37 degrees C for 1 h to repair DNA breaks. Thirty-five woodworkers and 41 control subjects were studied. In the woodworkers, significantly more cells with DNA damage (21.5%) were observed than in the control persons (9.7%). A slight but significant decrease in the level of DNA damage was found in the WBC of woodworkers after incubation (17.2%). Significantly higher levels of damaged DNA was observed in woodworkers who either smoked (22.1%) or did not smoke cigarettes (20.8%) than in smokers (13.2%) and non-smokers (7.0%) from the control group. After incubation, a slight decrease in the level of DNA damage was found in both smoking and non-smoking woodworkers compared to the respective subjects in the control group. The increased levels of DNA damage observed in the woodworkers could be associated with the occupational exposure to wood dust in the furniture manufacture.
该研究旨在基于白细胞（WBC）中的DNA损伤水平，评估木制家具制造工人的遗传毒性效应。采用单细胞碱性凝胶电泳试验（即彗星试验）检测白细胞中的受损DNA。DNA损伤水平通过出现彗星的细胞百分比来确定。在37℃的RPMI 1640培养基和二氧化碳环境中孵育1小时以修复DNA断裂前后，对细胞进行了评估。研究了35名木工和41名对照对象。在木工中，观察到DNA损伤的细胞（21.5%）明显多于对照人员（9.7%）。孵育后，木工白细胞中的DNA损伤水平略有但显著下降（17.2%）。与对照组中的吸烟者（13.2%）和非吸烟者（7.0%）相比，吸烟（22.1%）或不吸烟（20.8%）的木工中观察到的受损DNA水平明显更高。孵育后，与对照组中的相应对象相比，吸烟和不吸烟的木工的DNA损伤水平均略有下降。在木工中观察到的DNA损伤水平增加可能与家具制造中职业性接触木尘有关。
