Palus J, Dziubałtowska E, Rydzyński K
Nofer Institute of Occupational Medicine, Department of Toxicology and Carcinogenesis, Lódź, Poland.
Mutat Res. 1998 Aug 7;408(2):91-101. doi: 10.1016/s0921-8777(98)00021-4.
DNA single-strand breaks (DNA SSB) in peripheral lymphocytes of wooden furniture workers and a control group, including smokers and nonsmokers, were detected by the microfiltration method. Our results show that cigarette smoking significantly increases the fragmentation of DNA single strands in the wooden furniture workers (by nearly two times) but not in the control group. Moreover, occupational exposure to wood dust and other wooden plant substances significantly induced DNA SSB only in the lymphocytes of smokers (by about two times). DNA repair in the lymphocytes was investigated as 3H incorporation into DNA. High 3H incorporation in the unstimulated lymphocytes of both smoking and nonsmoking workers, as compared to the references, suggests that besides DNA SSB other DNA damage can be caused by occupational exposure in the wooden plant. Since DNA repair is not always perfect, the possibility is high that the low level of DNA repair in the study group may lead to irreversible DNA damage. We think that the workers exposed to wood dust and the substances emitted by furniture coating materials in the plant may be at higher risk for mutagenesis and/or carcinogenesis than the unexposed population.
采用微滤法检测了木制家具工人以及包括吸烟者和非吸烟者在内的对照组外周血淋巴细胞中的DNA单链断裂（DNA SSB）情况。我们的结果表明，吸烟显著增加了木制家具工人DNA单链的断裂程度（增加了近两倍），但在对照组中未出现这种情况。此外，职业性接触木尘和其他木制植物物质仅在吸烟者的淋巴细胞中显著诱导了DNA SSB（增加了约两倍）。通过检测淋巴细胞中3H掺入DNA的情况来研究淋巴细胞中的DNA修复。与参考组相比，吸烟和不吸烟工人未受刺激的淋巴细胞中3H掺入率较高，这表明除了DNA SSB外，职业性接触木制植物还可能导致其他DNA损伤。由于DNA修复并不总是完美的，研究组中DNA修复水平较低很可能会导致不可逆的DNA损伤。我们认为，在工厂中接触木尘和家具涂料材料释放的物质的工人，可能比未接触者有更高的诱变和/或致癌风险。
