Bremer S, Vogel R
Department of Chemicals Assessment, Federal Institute for the Health Protection of Consumers and Veterinary Medicine (BgVV), PO Box 33 00 13, 14191, Berlin, Germany.
Mutat Res. 1999 Jul 21;444(1):97-102. doi: 10.1016/s1383-5718(99)00090-x.
We tried to develop an in-vitro test system which could serve as a model for mammalian germ cells in vivo. Two pluripotent cell types were used, because they express some germ cell specific immunological and biochemical markers: (1) Embryonal carcinoma cells (ECC) of the line P19 had been isolated from a teratocarcinoma of murine primordial germ cells (PGC). (2) Embryonal stem cells (ESC) are obtained from the inner cell mass of mouse blastocysts. Sister chromatid exchanges (SCE) induced by mitomycin C and ethylnitrosourea (ENU) were analysed in the two undifferentiated cell lines, ECC and ESC, to detect differences in their sensitivity compared with differentiated cell lines of the mouse. Neither of the model cell lines have shown a greater sensibility after exposure to MMC and ENU. In contrary, the carcinoma cell line was able to tolerate higher concentrations of these genotoxicants. Therefore, SCE analysis in the ECC and ESC lines used does not provide a suitable model for genotoxicity testing on mammalian germ cells.
我们试图开发一种体外测试系统，该系统可作为体内哺乳动物生殖细胞的模型。我们使用了两种多能细胞类型，因为它们表达一些生殖细胞特异性免疫和生化标记：（1）P19系胚胎癌细胞（ECC）是从小鼠原始生殖细胞（PGC）的畸胎瘤中分离出来的。（2）胚胎干细胞（ESC）取自小鼠囊胚的内细胞团。分析了丝裂霉素C和乙基亚硝基脲（ENU）诱导的姐妹染色单体交换（SCE）在两种未分化细胞系ECC和ESC中的情况，以检测它们与小鼠分化细胞系相比的敏感性差异。在暴露于MMC和ENU后，这两种模型细胞系均未表现出更高的敏感性。相反，癌细胞系能够耐受更高浓度的这些基因毒性剂。因此，在所使用的ECC和ESC系中进行的SCE分析并不能为哺乳动物生殖细胞的遗传毒性测试提供合适的模型。
