Nussler A K, Vergani G, Gollin S M, Dorko K, Morris S M, Demetris A J, Nomoto M, Beger H G, Strom S C
Department of General Surgery, University of Ulm, Germany.
In Vitro Cell Dev Biol Anim. 1999 Apr;35(4):190-7. doi: 10.1007/s11626-999-0026-4.
The isolation and characterization of human liver cell lines are rather difficult due to limited material and poor growth in cell culture. In this report, we present the isolation, culture and characterization of a new epithelial-like liver cell line (AKN-1) with a heterogeneous cell population and many characteristics of the biliary epithelium. The AKN-1 cell line stained positively with antibodies to epithelial cytokeratin polypetides CK 8, 18, and 19. In addition, the cell line expressed the anti-human epithelial-related antigen (MOC-31), the human epithelial antigen (HEA), and the gamma-glutamyl transpeptidase, the hematopoietic growth factor, stem cell factor, and also its receptor, c-kit. The cell line failed to express albumin and factor 8 by immunohistochemistry. It did show, however, a twofold increase in 7-ethoxyresorufin-O-deethylase activity. Cytogenetic characterization revealed rare breakpoints in chromosome 2, which to our knowledge, have not yet been reported in liver cells.
由于材料有限以及细胞培养中生长不佳，人肝细胞系的分离和鉴定相当困难。在本报告中，我们展示了一种新的上皮样肝细胞系（AKN-1）的分离、培养和鉴定，该细胞系具有异质性细胞群体以及许多胆管上皮的特征。AKN-1细胞系用抗上皮细胞角蛋白多肽CK 8、18和19的抗体染色呈阳性。此外，该细胞系表达抗人上皮相关抗原（MOC-31）、人上皮抗原（HEA）以及γ-谷氨酰转肽酶、造血生长因子、干细胞因子及其受体c-kit。通过免疫组织化学，该细胞系未能表达白蛋白和因子8。然而，它确实显示出7-乙氧基异吩恶唑酮-O-脱乙基酶活性增加了两倍。细胞遗传学鉴定揭示了染色体2中罕见的断点，据我们所知，这在肝细胞中尚未见报道。
