Kumamoto H, Ooya K
Department of Oral Pathology, Tohoku University School of Dentistry, Sendai, Japan.
J Oral Pathol Med. 1999 Sep;28(8):343-9. doi: 10.1111/j.1600-0714.1999.tb02051.x.
To study the possible roles of bcl-2 family proteins in oncogenesis and cytodifferentiation of odontogenic epithelium, the expression of bcl-2, bcl-x and bax proteins was examined immunohistochemically in tooth germs and various types of ameloblastoma. In ameloblastomas, bcl-2 and bcl-x proteins were expressed intensely in peripheral columnar cells and weakly in central polyhedral cells, while bax protein reactivity was low. In variants of ameloblastoma, no expression of these proteins was found in the keratinizing areas or granular cell clusters. Basal cell ameloblastomas showed intense reactivity for bcl-2 and bcl-x proteins, but slight or no reactivity for bax protein. These results indicate that in ameloblastomas the bcl-2 family proteins might function primarily as anti-apoptotic factors, reflecting proliferative activity. Malignant ameloblastomas showed decreased reactivity for bcl-2 protein and increased levels of bax protein as compared with benign ones.
为研究bcl-2家族蛋白在牙源性上皮肿瘤发生及细胞分化中的可能作用，采用免疫组织化学方法检测了bcl-2、bcl-x及bax蛋白在牙胚和各种类型成釉细胞瘤中的表达。在成釉细胞瘤中，bcl-2和bcl-x蛋白在周边柱状细胞中强表达，在中央多角形细胞中弱表达，而bax蛋白反应性较低。在成釉细胞瘤的变异型中，在角化区或颗粒细胞团中未发现这些蛋白的表达。基底细胞型成釉细胞瘤对bcl-2和bcl-x蛋白显示强反应性，但对bax蛋白显示轻微反应或无反应。这些结果表明，在成釉细胞瘤中，bcl-2家族蛋白可能主要作为抗凋亡因子发挥作用，反映增殖活性。与良性成釉细胞瘤相比，恶性成釉细胞瘤对bcl-2蛋白的反应性降低，bax蛋白水平升高。
