Ostrowsky B E, Clark N C, Thauvin-Eliopoulos C, Venkataraman L, Samore M H, Tenover F C, Eliopoulos G M, Moellering R C, Gold H S
Department of Medicine, Division of Infectious Diseases, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.
J Infect Dis. 1999 Oct;180(4):1177-85. doi: 10.1086/315030.
VanD-mediated glycopeptide resistance has been reported for an isolate of Enterococcus faecium, BM4339. Three clinical isolates of vancomycin-resistant E. faecium collected from 3 patients during a 6-week period in 1993 had agar dilution MICs of vancomycin and teicoplanin of 128 and 4 microg/mL, respectively. Polymerase chain reaction (PCR) using degenerate primers complementary to genes encoding d-Ala-d-X ligases yielded a 630-bp product that was similar to the published partial sequence of vanD. By use of inverse PCR, vanD, vanHD, and two partial flanking open-reading frames were sequenced. The deduced amino acid sequence of VanD showed 67% identity with VanA and VanB. vanD appeared to be located on the chromosome and was not transferable to other enterococci. The 3 isolates were indistinguishable by pulsed-field gel electrophoresis and differed from BM4339. No other isolates carrying vanD were found in a subset of 875 recent US isolates of vancomycin-resistant enterococci.
已报道粪肠球菌分离株BM4339存在VanD介导的糖肽类耐药性。1993年，在6周内从3名患者中收集的3株耐万古霉素粪肠球菌临床分离株，其万古霉素和替考拉宁的琼脂稀释法最低抑菌浓度（MIC）分别为128和4μg/mL。使用与编码d-Ala-d-X连接酶的基因互补的简并引物进行聚合酶链反应（PCR），得到一个630 bp的产物，该产物与已发表的vanD部分序列相似。通过反向PCR对vanD、vanHD和两个侧翼部分开放阅读框进行了测序。推导的VanD氨基酸序列与VanA和VanB的一致性为67%。vanD似乎位于染色体上，不能转移到其他肠球菌。这3株分离株通过脉冲场凝胶电泳无法区分，且与BM4339不同。在最近美国的875株耐万古霉素肠球菌分离株中，未发现携带vanD的其他分离株。
