Tsutsumi S, Rokutan K, Tsuchiya T, Mizushima T
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Biol Pharm Bull. 1999 Aug;22(8):886-7. doi: 10.1248/bpb.22.886.
We recently found that guinea pig gastric pit cells in culture undergo spontaneous and rapid apoptotic DNA fragmentation, which may represent the rapid death of gastric pit cells in vivo. In this study, we observed that pretreatment of cells with geranylgeranylacetone, an antiulcer drug with heat-shock protein-inducing activity, suppressed the spontaneous apoptotic DNA fragmentation in a dose-dependent manner. Pretreatment of cells with low concentrations of ethanol or heat-shock also prevented the spontaneous apoptotic DNA fragmentation. These observations indicate that the suppression of the apoptotic DNA fragmentation by geranylgeranylacetone involve the induction of heat-shock proteins.
我们最近发现，培养的豚鼠胃小凹细胞会发生自发性快速凋亡性DNA片段化，这可能代表了体内胃小凹细胞的快速死亡。在本研究中，我们观察到，用具有热休克蛋白诱导活性的抗溃疡药物香叶基香叶基丙酮预处理细胞，可剂量依赖性地抑制自发性凋亡性DNA片段化。用低浓度乙醇或热休克预处理细胞也可防止自发性凋亡性DNA片段化。这些观察结果表明，香叶基香叶基丙酮对凋亡性DNA片段化的抑制作用涉及热休克蛋白的诱导。
