Bateman S W, Mathews K A, Abrams-Ogg A C, Lumsden J H, Johnstone I B
Department of Clinical Studies, Ontario Veterinary College, University of Guelph.
Can J Vet Res. 1999 Jul;63(3):216-20.
Freezing is a routine method of storage for plasma that is to be used in evaluating certain aspects of hemostatic function in many species. The purpose of this study was to evaluate the effect of storage at -70 degrees C for 6 mo on canine plasma samples. On fresh and frozen plasma from 12 clinically healthy dogs, prothrombin time, activated partial thromboplastin time, thrombin clotting time, fibrinogen determination, antithrombin III activity, fragment D and E assay, and protamine sulfate test were performed. Clinical agreement analysis was utilized to determine the effect of such storage on all assays. Individual differences detected between fresh and frozen samples were all within 2 standard deviations of the mean difference. With the exception of the activated partial thromboplastin time, storing canine plasma at -70 degrees C for 6 mo has no significant effect on hemostatic function, as assessed by these tests.
冷冻是血浆储存的常规方法，在许多物种中用于评估止血功能的某些方面。本研究的目的是评估在-70℃储存6个月对犬血浆样本的影响。对12只临床健康犬的新鲜和冷冻血浆进行了凝血酶原时间、活化部分凝血活酶时间、凝血酶凝血时间、纤维蛋白原测定、抗凝血酶III活性、D和E片段测定以及硫酸鱼精蛋白试验。采用临床一致性分析来确定这种储存对所有检测的影响。新鲜样本和冷冻样本之间检测到的个体差异均在平均差异的2个标准差范围内。通过这些检测评估，除活化部分凝血活酶时间外，将犬血浆在-70℃储存6个月对止血功能没有显著影响。
