Bortnick S J, Orandle M S, Papadi G P, Johnson C M
Division of Comparative Medicine, College of Veterinary Medicine, University of Florida, Gainesville 32610-0880, USA.
Lab Anim Sci. 1999 Aug;49(4):395-400.
To compare lymphocyte subpopulations in the blood and lymphoid tissues of normal kittens between 1 and 90 days of age.
Lymphocyte subsets within the blood, thymus, and lymph node of 24 normal kittens were quantified by use of two-color fluorescence flow cytometry and were compared at 1, 23, 46, or 90 days after birth.
Blood B and T lymphocytes increased over the 90-day postnatal period. The CD4+ and CD8+ sub-populations of T lymphocytes increased. However, CD8+ lymphocytes increased more than did CD4+ lymphocytes, resulting in reduced CD4-to-CD8 ratio. By 23 days of age, similar but more abrupt changes in the CD4-to-CD8 ratio occurred in the thymus and lymph nodes, coinciding with the highest thymus-to-body weight ratio and gradual increase in mature thymocytes expressing a pan-T lymphocyte marker.
Postnatal thymopoiesis in the domestic cat favors production of mature CD8+ T lymphocytes over CD4+ T lymphocytes. This coincides with the emergence of CD8+ lymphocytes in the lymph node and precedes a more gradual increase in CD8+ cells in the blood. Therefore, the ontogeny of these effectors of cell-mediated immunity could be interrupted by infective agents that target lymphoid tissues of the neonate.
比较1至90日龄正常小猫血液和淋巴组织中的淋巴细胞亚群。
使用双色荧光流式细胞术对24只正常小猫血液、胸腺和淋巴结内的淋巴细胞亚群进行定量，并在出生后1、23、46或90天进行比较。
出生后90天内，血液中的B淋巴细胞和T淋巴细胞增多。T淋巴细胞的CD4+和CD8+亚群增加。然而，CD8+淋巴细胞的增加幅度大于CD4+淋巴细胞，导致CD4与CD8比值降低。到23日龄时，胸腺和淋巴结中的CD4与CD8比值出现类似但更突然的变化，这与胸腺与体重比值最高以及表达泛T淋巴细胞标志物的成熟胸腺细胞逐渐增加相一致。
家猫出生后的胸腺生成更倾向于产生成熟的CD8+ T淋巴细胞而非CD4+ T淋巴细胞。这与淋巴结中CD8+淋巴细胞的出现相一致，并先于血液中CD8+细胞的逐渐增加。因此，这些细胞介导免疫效应器的个体发生可能会被针对新生儿淋巴组织的感染因子所中断。
